重組乳酸脫氫酶B/LDHB
產品編號：ENZ-279
產品規(guī)格：1mg/3mg/30mg
產品規(guī)格：1mg/3mg/30mg
| Synonyms | Lactate Dehydrogenase, LDH. |
| Introduction | Lactate dehydrogenase （LDH） is an enzyme（EC1.1.1.27） present in a wide variety of organisms, including plants and animals. A tetrameric enzyme that catalyses the interconversion of pyruvateand lactatewith concomitant interconversion of NADH and NAD+. At high concentrations of pyruvate, the enzyme exhibits feedback inhibition and the rate of conversion of pyruvate to lactate is decreased. In vertebrates, genes for three different subunits （LDH-A, LDH-B and LDH-C） exist. |
| Description | The DNA encoding chicken LDH-B is cloned from cDNA library of chicken heart. |
| Source | Escherichia Coli. |
| Physical Appearance | Sterile lyophilized powder. |
| Formulation | The protein （1 mg/ml） was lyophilized with 0.1mg potassium phosphate. |
| Solubility | It is recommended to reconstitute the lyophilized LDH in sterile 18MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. |
| Stability | Lyophilized Lactate Dehydrogenase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution LDH should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles. |
| Purity | Greater than 95.0% as determined by（a） Analysis by RP-HPLC. （b） Analysis by SDS-PAGE. |
| Biological Activity | The specific activity was found to be 258 U/mg protein. |
| Unit Definition | One unit is defined as 1umol of NAD+ production per minute under the assay conditions （25°C, pH 7.0）. Both transaminase activities include a-hydroxyglutarate dehydrogenase activity. |
| Usage | Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
