Catalogue Number	ENZ-266
Synonyms	Polyphosphate kinase, EC 2.7.4.1, Polyphosphoric acid kinase, ATP-polyphosphate phosphotransferase.
Introduction	PPK catalyzes the reversible transfer of phosphate between polyphosphate and ATP. The phosphorylation of ADP to ATP by polyphosphate kinase is by a processive mechanism; the phosphorylation occurs without release of the polymer from the enzyme prior to termination of the reaction.
Description	Polyphosphate Kinase purified circa 10 fold . Free of all Polyphosphate Glucokinase activity. Native MW = 83 kD （monomer）.
Source	Propionibacterium shermanii.
Physical Appearance	Sterile Filtered White lyophilized （freeze-dried） powder.
Formulation	The protein was lyophilized from 1.54ml PPK solution containing 32.45U/ml of PPK activity, 3.87mg/ml total protein, 10mM potassium phosphate pH 6.8 and 25mM sodium polyphosphate.
Solubility	The contents of the vial can be reconstituted in 1-10 ml deionized water.
Stability	Lyophilized Polyphosphate kinase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PPK should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles.
Definition of Activity Units	The amount of Polyphosphate kinase required to convert 1 μmole ADP to ATP per minute at pH 7.5, using polyphosphate as phosphate donor.
Specific Activity	8.39 U/mg.
Required Cofactors	The reaction requires Mg2+.
Usage	Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.