重組白血病病毒反轉錄酶/MMLV RT
產品編號：ENZ-310
產品規格：2500U/10000U/50000U
產品規格：2500U/10000U/50000U
| Catalogue Number | ENZ-310 |
| Description | MMLV （Moloney Murine Leukemia Virus） Reverse Transcriptase is a DNA polymerase that synthesizes a complementary DNA strands from single-stranded RNA, DNA, or an RNA-DNA hybrid as a template. This recombinant enzyme was purified from E.coli, which carried a modified MMLV-RT gene. Compared to AMV Reverse Transcriptase, this enzyme has a much weaker 5' - 3' ribonuclease H activity, which allows the syntesis of longer cDNAs （>7kb）. |
| Source | Recombinant E. coli strain. |
| Physical Appearance | Sterile Filtered clear solution （200 U/μl）. |
| Concentration | 200 units/ml. |
| Formulation | 50mM Tris-HCl, 0.1M NaCl, 0.1% Triton X-100, 2mM DTT, 0.1mM EDTA and 50% glycerol. |
| Stability | Stable for 5 days at 10°C, for longer period of time store at -20°C. Please prevent freeze-thaw cycles. |
| Applications | 1. 1st strand cDNA synthesis. 2. Primer extension. 3. RT-PCR. |
| Unit Definition | One unit is defined as the amount of enzyme required to catalyze the incorporation of 1nmol of deoxyribonucleotide into acid-insoluble forms in 10 minutes at 37oC, using poly（A）-oligo（dT）12-18 as the template-primer. Standard cDNA Synthesis Conditions50mM Tris-HCl （pH8.3）, 75mM KCl, 3mM MgCl2, 10mM DTT, 1.0mM each dATP, dGTP, dCTP, and dTTP, 0.2 mg radom hexamer,1-5mg RNA, 200units M-MLV RT. The reaction volume was 20ml and the incubation was 45 min at 42oC. |
| 5X Reaction Buffer | 250mM Tris-HCl （pH8.3）, 375mM KCl, 15mM MgCl2, and 50mM DTT. |
| Usage | Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
