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廣電計量檢測集團股份有限公司

GRO1/KC Mouse/ 重組鼠GRO1/KC

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  • 公司名稱廈門慧嘉生物科技有限公司
  • 品       牌
  • 型       號
  • 所  在  地廈門市
  • 廠商性質代理商
  • 更新時間2016/1/22 2:05:05
  • 訪問次數259
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代理和經銷眾多生命科學領域的。公司以價格優、到貨快,反應及時,服務周到,誠信交易為宗旨與理念,致力于各種生物試劑、抗體、試劑盒、耗材、儀器的銷售推廣及服務工作。另外,還備有常規生化試劑、抗體、分子生物學試劑盒.

ELISA試劑盒、抗體、生化試劑、抗體、細胞因子、重組蛋白、耗材
GRO1/KC Mouse/ 重組鼠GRO1/KC 產品編號:CHM-335
產品規格:5μg/20μg/1mg
GRO1/KC Mouse/ 重組鼠GRO1/KC 產品信息

GRO1/KC Mouse/ 重組鼠GRO1/KC

產品編號:CHM-335
產品規格:5μg/20μg/1mg
 

Synonyms Growth-regulated alpha protein, CXCL1, Plaet-derived growth factor-inducible protein KC, Secretory protein N51, KC, Fsp, N51, gro, Gro1, Mgsa, Scyb1, chemokine (C-X-C motif) ligand 1.
Introduction Chemokine (C-X-C motif) ligand 1 (CXCL1) is a small cytokine belonging to the CXC chemokine family that was previously called GRO1 oncogene, Neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-?). It is secreted by human melanoma cells, has mitogenic properties and is implicated in melanoma pathogenesis. CXCL1 is expressed by macrophages, neutrophils and epithelial cells, and has neutrophil chemoattractant activity. CXCL1 plays a role in spinal cord development by inhibiting the migration of oligodendrocyte precursors and is involved in the processes of angiogenesis, inflammation, wound healing, and tumorigenesis. This chemokine elicits its effects by signaling through the chemokine receptor CXCR2. The gene for CXCL1 is located on human chromosome 4 amongst genes for other CXC chemokines.
Description KC Mouse Recombinant also known as N51 and GRO1 produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 77 amino acids and having a molecular mass of approximay 8 kDa.
The GRO-1 is purified by proprietary chromatographic techniques.
Source Escherichia Coli.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation The protein was lyophilized with no additives.
Solubility It is recommended to reconstitute the lyophilized GRO1 Recombinant in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.
Stability Lyophilized KC Mouse although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CXCL1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity Greater than 95.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Amino acid sequence RLATGAPIANELRCQ CLQTMAGIHL KNIQSLKVLP SGPHCTQTEV IATLKNGREA CLDPEAPLVQ KIVQKMLKGV PK.
Biological Activity The biological activity was determined by measuring the dose dependent mobilization of intracellular calcium (calcium flux) with human neutrophils. Significant calcium mobilization is observed with ?50ng/mL of recombinant mouse KC (Specific Activity: ? 2 x 104 units/mg).
Protein content Protein quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 0.03 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (InliGenetics).

2. Analysis by RP-HPLC, using a standard solution of KC as a Reference Standard.
Usage ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

15168338725
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