| Catalogue Number | CYT-033 | ||
| Synonyms | Interleukin-21, IL-21, Il21. | ||
| Introduction | IL-21 is produced by CD4+ T cells in response to antigenic stimulation. Its action enhances antigen-specific responses of immune cells. The biological effects of IL-21 include induction of differentiation of T-cells-stimulated B-cells into plasma cells and memory B-cells, stimulation （in conjuction） with IL-4 of IgG production, and induction of apoptotic effects in naive B-cells and stimulated B-cells in the absence of T-cell signaling. Additionally, IL-21 promotes the anti-tumor activity of CD8+ T-cells and NK cells. IL-21 exerts its effect through binding to a specific type I cytokine receptor, IL-21R, which also contains the gamma chain （°C） found in other cytokine receptors including IL-2, IL-4, IL-7, IL-9 and IL-15. The IL-21/IL-21R interaction triggers a cascade of events which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3. | ||
| Description | Interleukin-21 Rat Recombinant produced in E.Coli is a single, non-glycosilated polypeptide chain containing 129 amino acids and having a total molecular mass of 15.2kDa. The Rat IL-21 is purified by proprietary chromatographic techniques. | ||
| Source | Escherichia Coli. | ||
| Physical Appearance | Sterile Filtered White lyophilized （freeze-dried） powder. | ||
| Formulation | Rat IL-21 was lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4. | ||
| Solubility | It is recommended to reconstitute the lyophilized Rat IL21 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | ||
| Stability | Lyophilized Rat IL-21 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Rat IL21 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles. | ||
| Purity | Greater than 95.0% as determined by: （a） Analysis by RP-HPLC. （b） Analysis by SDS-PAGE. | ||
| Amino acid sequence | HKSSPQRPDH LLIRLRHLMD IVEQLKIYEN DLDPELLTAP QDVKGQCEHE AFACFQKAKL KPSNTGNNKT FINDLLAQLR RRLPAKRTGN KQRHMAKCPS CDLYEKKTPK EFLERLKWLL QKMIHQHLS. | ||
| Biological Activity | The biological activity is measured by its ability to proliferate activated B cells. | ||
| Usage | ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. | ||
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