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重組大鼠白介素18,Rat IL-18

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  • 公司名稱上海高創化學科技有限公司
  • 品       牌
  • 型       號5ug
  • 所  在  地上海市
  • 廠商性質代理商
  • 更新時間2016/1/3 0:57:26
  • 訪問次數718
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上海高創化學科技有限公司是一家專注于生命科學和生物技術領域的高科技企業,是國內代理銷售生物試劑、儀器的公司之一。公司本著為顧客服務、為科研服務的宗旨,為中國的專家和學者提供種類齊全的高品質產品。高創公司已與多家歐美*生物技術公司簽訂*或區域代理協議,銷售的實驗儀器、消耗品、分子生化試劑、免疫試劑和細胞培養試劑等,已被廣泛應用于生命科學基礎研究、開發應用、制藥、疾病診斷與控制、人口與健康、生物技術等諸多領域。客戶遍布大學、研究所、醫院、衛生防疫、商品檢驗檢疫、制藥公司、生物技術公司和食品工業等單位。主要產品包括:英國Randox-lifesciences公司的藥物殘留ELISA試劑盒、抗體抗體蛋白;挪威Biosese公司的檢測表面活性劑、農藥殘留、毒素、PCB等相關有害物質的ELISA檢測試劑盒以及魚類蛋白和抗體;Gibco公司DMEM培養基、1640培養基、F12培養基,MEM培養基、F10培養基等;Prospec-Tany公司的細胞因子、生長因子、蛋白激酶、重組和天然蛋白、酶以及病毒抗原和抗體;美國eBioscience公司的流式細胞術產品等。
 

高創的宗旨是:為用戶提供“Z高質量的產品” 和 “*質的服務”

ELISA試劑盒,細胞因子,重組蛋白,抗原,抗體,生化試劑,分子生物學試劑
Recombinant Rat Interleukin-18
重組大鼠白介素18
Rat IL-18
產品編號:cyt-376
重組大鼠白介素18,Rat IL-18 產品信息
Catalogue Number CYT-376
Patent Protected Countries The Sale of recombinant Rat IL-18 by ProSpec is prohibited in the following countries: United States, Japan, United Kingdom, France, Spain, Italy, Germany, Austria, Belgium, Denmark, Portugal, Sweden, Switzerland, Liechtenstein, Australia, Taiwan, Netherlands, Korea and Canada.
Synonyms Interferon-gamma-inducing factor, IGIF, IL-1g, IL-18, IL1F4, MGC12320, IFN-gamma-inducing factor, Interleukin-1 gamma, IL-1 gamma, Iboctadekin.
Introduction IL-18 is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity.
Description Interleukin-18 Rat Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 158 amino acids and having a molecular mass of 18260 Dalton.
The IL-18 is purified by proprietary chromatographic techniques.
Source Escherichia Coli.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a concentrated (1mg/ml) solution in water containing 0.4 x PBS pH=7.2.
Solubility It is recommended to reconstitute the lyophilized Interleukin 18 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability Lyophilized Interleukin-18 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL18 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity Greater than 95.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Amino acid sequence The sequence of the first five N-terminal amino acids was determined and was found to be His-Phe-Gly-Arg-Leu.
Biological Activity The ED50 as determined by the dose-dependant stimulation of IFN-gamma by murine lymph node cells (400,000cells/well) co-stimulated with sub optimal dose of ConA (0.75ug/ml) is 12ng/ml corresponding to a specific activity of 84,000U/mg. The medium was RPMI containing 10% FCS and 50µM 2-ME.
Protein content Protein quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 0.213 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (InliGenetics).

2. Analysis by RP-HPLC, using a standard solution of IL-18 as a Reference Standard.
Usage ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Related Products
» IL 18 Human
» IL 18 Human, His
» IL 18 Mouse
» IL18 Antibody

 

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