| Catalogue Number | CYT-442 | ||||
| Synonyms | Cartilage-derived morphogenetic protein-1, CDMP-1, LAP4, SYNS2, GDF-5, Radotermin, CDMP1, GDF5, Growth differentiation factor 5, BMP-14. | ||||
| Introduction | GDF-5 is a member of the bone morphogenetic protein （BMP） family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Mutations in this gene are associated with acromesomelic dysplasia, Hunter-Thompson type; brachydactyly, type C; and chondrodysplasia, Grebe type. These associations confirm that the gene product plays a role in skeletal development. | ||||
| Description | Growth Differentiation Factor 5 Human Recombinant produced in E.Coli is a homodimer, non-glycosylated, polypeptide chain containing 2 x 120 amino acids and having a total molecular mass of 26.8 kDa. GDF5 is purified by proprietary chromatographic techniques. | ||||
| Source | Escherichia Coli. | ||||
| Physical Appearance | Sterile Filtered White lyophilized （freeze-dried） powder. | ||||
| Formulation | The protein was lyophilized without any additives. | ||||
| Solubility | It is recommended to reconstitute the lyophilized Growth Differentiation Factor-5 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | ||||
| Stability | Lyophilized Growth Differentiation Factor 5 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Growth Differentiation Factor-5 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles. | ||||
| Purity | Greater than 98.0% as determined by: （a） Analysis by RP-HPLC. （b） Analysis by SDS-PAGE. | ||||
| Biological Activity | GDF-5 activity was determined by the induction of alkaline phosphatase activity in ATDC5 cells: EC50 = 4ug/ml, corresponding to a specific activity of 250units/mg. | ||||
| Amino Acid Sequence | APSATRQGKR PSKNLKARCS RKALHVNFKD MGWDDWIIAP LEYEAFHCEG LCEFPLRSHL EPTNHAVIQT LMNSMDPEST PPTCCVPTRL SPISILFIDS ANNVVYKQYE DMVVESCGCR. | ||||
| Protein content | Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 1.15 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）. 2. Analysis by RP-HPLC, using a standard solution of GDF5 as a Reference Standard. | ||||
| References | Title:Growth/differentiation Factor-5 Induces Osteogenic Differentiation of Human Ligamentum Flavum Cells through Activation of ERK1/2 and p38 MAPK. Publication:Cell Physiol Biochem 2010;26:179-186 （DOI: 10.1159/000320526） Link:http://content.karger.com/produktedb/produkte.asp?doi=320526 | ||||
| Usage | ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. | ||||
| Related Products |
|
上海高創化學科技有限公司，是一家專注于生命科學和生物技術領域的高科技企業，是國內代理銷售生物試劑、儀器的公司之一。公司本著為顧客服務、為科研服務的宗旨，為中國的專家和學者提供種類齊全的高品質產品。高創公司已與多家歐美*生物技術公司簽訂*或區域代理協議，銷售的實驗儀器、消耗品、分子生化試劑、免疫試劑和細胞培養試劑等，已被廣泛應用于生命科學基礎研究、開發應用、制藥、疾病診斷與控制、人口與健康、生物技術等諸多領域?？蛻舯椴即髮W、研究所、醫院、衛生防疫、商品檢驗檢疫、制藥公司、生物技術公司和食品工業等單位。主要產品包括：英國Randox-lifesciences公司的藥物殘留ELISA試劑盒、抗體抗體蛋白；挪威Biosese公司的檢測表面活性劑、農藥殘留、毒素、PCB等相關有害物質的ELISA檢測試劑盒以及魚類蛋白和抗體；Gibco公司DMEM培養基、1640培養基、F12培養基，MEM培養基、F10培養基等；Prospec-Tany公司的細胞因子、生長因子、蛋白激酶、重組和天然蛋白、酶以及病毒抗原和抗體；美國eBioscience公司的流式細胞術產品等。
高創的宗旨是：為用戶提供“Z高質量的產品” 和 “*質的服務”。
ELISA試劑盒，細胞因子，重組蛋白，抗原，抗體，生化試劑，分子生物學試劑
重組人生長分化因子5,Human GDF5 產品信息
