產品名稱：Halt Protease & Phosphatase Inhibitor Cocktail
貨號：Pierce 78440
規格：1ML
The all-in-one Thermo Scientific Halt Protease and Phosphatase Inhibitor Cocktails provide the convenience of a single solution with full protein sample protection for cell and tissue lysates.
This broad-spectrum Halt Protease and Phosphatase Inhibitor Cocktail is specifically optimized to protect proteins from degradation during extraction and purification. The cocktail contains inhibitors against the major classes of proteases and phosphatases, targeting aminopeptidases, cysteine and serine proteases and serine/threonine and protein tyrosine phosphatases. To promote the inhibition of metalloproteases, 0.5M EDTA is provided in a separate tube. The 100X cocktails, available in regular and EDTA-free formulations and several package formats, are refrigerator-stable liquids that are very easy to use for nearly any sample or experimental scale.
Highlights:
Complete protection – all-in-one cocktail contains both protease and phosphatase inhibitors
Multiple package sizes – choose from four convenient packages sizes to match nearly any scale
Convenient – the 100X liquid formulation is much easier and more effective to use than individual inhibitors and other formats
Easy storage – aqueous-based format allows for convenient cold-room storage without freezing, by contrast with DMSO-based cocktails
Compatible – can be used in mass spectrometry （MS） because it does not contain AEBSF, which can cause peaks to shift
Validated – fully compatible with Thermo Scientific Pierce Cell Lysis Buffers
Product Details:
This combined cocktail is the first of its kind. The aqueous-based format allows for convenient cold-room storage without freezing, which is associated with DMSO-based cocktails, and is much easier to use than combining individual inhibitors or using other formats. More importantly, the cocktail formulation is more effective than competing products for inhibiting proteases in lysates and preserving protein phosphorylation during cell harvest. The formulation is also MS-compatible because it does not contain AEBSF, which can cause peaks to shift.