產品名稱：EDC
貨號：Pierce 77149
規格：10 MG
Thermo Scientific Pierce EDC is a water-soluble carbodiimide crosslinker that activates carboxyl groups for spontaneous reaction with primary amines, enabling peptide immobilization and hapten-carrier protein conjugation.
1-Ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride （EDC or EDAC） is a zero-length crosslinking agent used to couple carboxyl groups to primary amines. This crosslinker has been used in diverse applications such as forming amide bonds in peptide synthesis, attaching haptens to carrier proteins to form immunogens, labeling nucleic acids through 5’ phosphate groups and creating amine-reactive NHS-esters of biomolecules. EDC reacts with a carboxyl to form an amine-reactive O-acylisourea intermediate. If this intermediate does not encounter an amine, it will hydrolyze and regenerate the carboxyl group. In the presence of N-hydroxysulfosuccinimide （Sulfo-NHS）, EDC can be used to convert carboxyl groups to amine-reactive Sulfo-NHS esters. This is accomplished by mixing the EDC with a carboxyl containing molecule and adding Sulfo-NHS.
Highlights:
Efficiency of EDC-mediated coupling is increased in the presence of Sulfo-NHS
By combining EDC and Sulfo-NHS, amine reactive Sulfo-NHS esters can be created on any carboxyl-containing molecule
Amide bonds formed with this crosslinking reaction provide a neutral linkage
Excess reagent and crosslinking byproducts are easily removed by washing with water or dilute acid
EDC is water soluble, so crosslinking can be done in physiologic solutions without adding organic solvent
High purity, crystalline EDC can be used to create high-purity activated derivatives
Product Details:
EDC reacts with a carboxyl group on molecule #1, forming an amine-reactive O-acylisourea intermediate. This intermediate may react with an amine on molecule #2, yielding a conjugate of the two molecules joined by a stable amide bond. However, the intermediate is also susceptible to hydrolysis, making it unstable and short-lived in aqueous solution. The addition of Sulfo-NHS （5 mM） stabilizes the amine-reactive intermediate by converting it to an amine-reactive Sulfo-NHS ester, thus increasing the efficiency of EDC-mediated coupling reactions1,2. The amine-reactive Sulfo-NHS ester intermediate has sufficient stability to permit two-step crosslinking procedures, which allows the carboxyl groups on one protein to remain unaltered.