產品名稱：Sample Krypton Protein Stain（10X）
貨號：Pierce 46628
規格：20ML
Thermo Scientific Krypton Fluorescent Protein Stain enables sensitive fluorescent visualization （520nm excitation; 580nm emission） of proteins separated by SDS-PAGE and on transfer membranes using popular commercial fluorescence imagers.
This fluorescent protein gel stain is for bright yellow-orange fluorescent detection of proteins separated by 1D or 2D polyacrylamide gel electrophoresis （SDS-PAGE）. Krypton Stain uses a protein-binding dye with fluorescence properties （Ex/Em = 520/580nm） suitable for visualization with a variety of standard laser-based and filter-based fluorescence imagers and documentation systems. Proteins are detectable at concentrations greater than 0.25ng per band in a typical mini-gel lane （greater than 2ng for the rapid staining protocol）. Krypton Stain provides high signal intensity, exhibits minimal protein-to-protein variation, has an excellent quantitative dynamic range and is compatible with subsequent destaining and analysis by mass spectrometry.
Intense fluorescent stain – excitation and emission maxima of 520nm and 580nm, respectively （compatible with 532nm excitation light sources and 580 to 600nm emission filters）
Instrument-compatible – effective with popular laser-based and filter-based fluorescence imagers
Broad dynamic range – linear quantitative range spans three to four orders of magnitude Sensitive – detect as little as 0.25ng protein with the standard protocol （approx. 2.5 hours）; at least as sensitive as fluorescent stains from other suppliers
Fast – standard （2.5 hours） and rapid （30 minutes） protocols are significantly faster than procedures of other popular fluorescent stains
Versatile – compatible with proteomics workflows involving downstream mass spectrometry
Compact – supplied as a 10X solution to save storage space; just dilute with water before use
Product Details:
For best results, detect bands using visible laser-based imagers equipped with a 532nm laser light source. Although 580nm-filters are optimal for emission, 600nm-filters are also compatible. Stained gels can be imaged on any platform with the respective excitation and emission filters.