產品名稱：Blocker Casein in PBS
貨號：Pierce 37528
規格：1 L
Thermo Scientific Blocker Casein Blocking Buffers are ready-to-use, PBS or TBS solutions of purified casein protein for blocking steps in Western blotting, ELISA, immunohistochemistry and nucleic acid detection methods.
These blocking buffers contains casein protein that is purified from milk by the Hammarsten method. The phosphate- and Tris-buffered saline formulations are used for blocking excess binding sites on membranes and microplates in antibody-based detection methods. Blocker Casein Buffers are 1% （w/v） casein, which corresponds to the optimal concentration for most applications.
Highlights:
Purified casein – single-protein blocking buffer provides fewer chances of cross-reaction with assay components than serum or milk solutions
Easy to use – 1% casein solutions are ready to use; can be diluted further as needed
Flexible – available in PBS and TBS formulations to suit a variety of applications
Safe – stable, thimerosal-free formulations
References:
Alegria-Schaffer, A., et al. （2009）. Performing and optimizing Western blots with an emphasis on chemiluminescent detection. Methods Enzymol. 463:573-99.
Nemzek, J.A. et al. （2000）. Am. J. Physiol. Lung Cell. Mol. Physiol. 278:L512-20.
Stuyver, L.J., et al. （2003）. Antimicrob. Agents Chemother. 47:244-254.
Wolfman, J.C., et al. （2002）. Mol. Cell. Biol. 22:1589-1606.