產品名稱：Lumi-Phos WB, 100 ML
貨號：Pierce 34150
規格：100ML
Thermo Scientific Lumi-Phos WB Substrate is a chemiluminescent substrate for sensitive detection of alkaline phosphatase activity from AP-conjugated antibodies and probes in Western blotting.
Lumi-Phos WB Chemiluminescent Substrate overcomes the limitations posed by conventional chemiluminescent substrates for alkaline phosphatase （AP）. Lumi-Phos WB Substrate provides Western blot detection of low-picogram （attomole） levels of protein targets, which is comparable in sensitivity to many ECL substrates for horseradish peroxidase （HRP）. Lumi-Phos WB Substrate also produces less background noise than other commercially available alk-phos chemiluminescent substrates, providing a better signal:noise ratio and a clearer image when exposed to autoradiography film or CCD imagers. Because signal generation is immediate, there's no need to wait 15 to 30 minutes for a measurable signal. All of these benefits are combined with an affordable price.
Highlights:
Alk-phos substrate – a chemiluminescent substrate for detection of alkaline phosphatase （AP or alk-phos） activity from antibodies and other Western blot probes
Highly sensitive – detect low-picogram amounts of protein targets; detected 1.2pg （71 attomoles） of mouse IL-2 in example experiment
Low background – provides high signal:noise ratios to yield clear blots that are easily documented by exposure to x-ray film or imagers
Inexpensive – less costly to use than CDP-Star Substrate （based on 2008 U.S. list prices）, and there is no need to purchase additional enhancers for nitrocellulose membranes
Long signal duration – when conditions are optimized, blots generate usable signal for up to 8 hours （Em-max equals 440nm）
Immediate strong signal – no more waiting 15 to 30 minutes for the signal to become strong enough to detect with imagers
Ready to use – no mixing required with this one-component system