產品名稱：TMB Substrate Kit
貨號：Pierce 34021
規格：400ML
Thermo Scientific Pierce TMB ELISA Substrates detect horseradish peroxidase （HRP） activity, yielding a blue color （Amax = 370nm and 652nm） that changes to yellow （Amax = 450nm） upon addition of a sulfuric or phosphoric acid stop solution.
TMB is the most popular chromogenic substrate for HRP detection in ELISA and is available in several formats. The Pierce 1-Step TMB Substrates are one-component substrates that require no preparation before use. Unlike other commercially available substrates, these products contain no DMF or DMSO.
Highlights:
Available in traditional or ready-to-use formulations
No additional reagents or filtering required
Non carcinogenic
Various levels of sensitivity and development speed to suit any assay
No DMF or DMSO present in the reagents
Product Details:
Thermo Scientific Pierce 1-Step Ultra TMB yields the greatest sensitivity among the TMB substrates, followed by 1-Step Turbo TMB and 1-Step Slow TMB. The sensitivity of the 1-Step Turbo TMB is similar to OPD-based substrates formulated at approximay 1mg/mL. 1-Step Slow TMB has intermediate sensitivity – more sensitive than ABTS, but less sensitive than o-phenylenediamine dihydrochloride （OPD） or 1-Step Turbo TMB. The 1-Step Slow TMB is an ideal substrate for kinetic studies.