產品名稱：Pierce Crosslink IP Kit
貨號：Pierce 26147
規格：kit
The Thermo Scientific Crosslink IP Kit extends the functionality of traditional immunoprecipitation （IP） methods by adding crosslinking technology and microcentrifuge spin column sample handling to the procedure.
The primary benefits resulting from these features are the ability to purify target protein without contamination by the antibody and the ability to more effectively wash and separate samples from the beaded agarose resin.
Highlights:
Eliminates antibody contamination – antibody is irreversibly attached to the agarose beads so that co-elution of heavy and light chains with the purified protein is minimized 
Easy and efficient scalability – use only the amount of antibody needed for a single IP experiment or immobilize 100-200 µg of antibody to prepare ready-made IP affinity resin for many experiments
Assay reliability and sample handling – Pierce Spin Columns eliminate resin loss and provide for more efficient separation of solutions than traditional IP methods that use only microcentrifuge tubes 
Prepared antibody affinity resin is reusable – because the antibody is covalently immobilized and usually is not inactivated by the mild elution procedure, the resin often can be used several times 
Complete kits – package includes sufficient reagents and spin columns to perform at least 50 antibody immobilizations and IP experiments
Applications:
Immunoprecipitation followed by electrophoresis and excision of the protein for mass spectrometry or sequencing identification analysis 
Immunoprecipitation and SDS-PAGE analysis of a target protein whose molecular weight is similar to the heavy or light chain antibody fragment 
Immunoprecipitation of target proteins for subsequent analysis by nondenaturing methods （i.e., methods not involving SDS-PAGE）