產品名稱：BCA Protein Assay Kit/BCA蛋白濃度測定試劑盒
貨號：Pierce 23225
規(guī)格：KIT
The Thermo Scientific Pierce BCA Protein Assay Kit remains one of the most popular protein quantitation methods worldwide.
Used in more labs than any other detergent-compatible protein assay, Pierce BCA Reagents provide accurate determination of protein concentration with most sample types encountered in protein research. The Pierce BCA Assay can be used to assess yields in whole cell lysates and affinity-column fractions, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA Assay is affected much less by protein compositional differences, providing greater protein-to-protein uniformity.
Highlights:
Colorimetric – estimate visually or measure with a standard spectrophotometer or plate reader （562nm）
Excellent uniformity – exhibits less protein-to-protein variation than dye-binding methods
Compatible – unaffected by typical concentrations of most ionic and nonionic detergents
Moderay fast – much easier and four times faster than the classical Lowry method
High linearity – linear working range for BSA equals 20 to 2000µg/mL
Sensitive – detect down to 5µg/mL with the enhanced protocol
BCA Protein Assay Applications:
Studying protein:protein interactions
Measuring column fractions after affinity chromatography
Estimating percent recovery of membrane proteins from cell extracts
High-throughput screening of fusion proteins