產品名稱：BS3, Bis（Sulfosuccinimidyl） Suberate
貨號：Pierce 21580
規格：50MG
Thermo Scientific Pierce BS3 （Sulfo-DSS） is bis（sulfosuccinimidyl）suberate, an amine-to-amine crosslinker that is homobifunctional, water-soluble, non-cleavable and membrane impermeable.
BS3 contains an amine-reactive N-hydroxysulfosuccinimide （NHS） ester at each end of an 8-carbon spacer arm. NHS esters react with primary amines at pH 7-9 to form stable amide bonds, along with release of the N-hydroxysulfosuccinimide leaving group. Proteins, including antibodies, generally have several primary amines in the side chain of lysine （K） residues and the N-terminus of each polypeptide that are available as targets for NHS-ester crosslinking reagents.
Because it contains the hydrophilic sulfonyl moiety, BS3 crosslinker is soluble up to ~10mM in water and many commonly used buffers, thus avoiding the use of organic solvents which may perturb protein structure. DSS, the non-water soluble analog of BS3 is also available for applications that require a less hydrophilic crosslinker （e.g., to effect intracellular crosslinking）. DSS and BS3 have essentially identical crosslinking activity toward primary amines.
BS3 Highlights
Amine-reactive Sulfo-NHS ester reacts rapidly with any primary amine-containing molecule
BS3 is water-soluble, so crosslinking can be done in physiologic solutions
Water-soluble crosslinking reagent is ideal for cell-surface crosslinking
High-purity, crystalline BS3 can be used to create high-purity crosslinked conjugates
Applications for BS3
Crosslink cell-surface proteins prior to cell lysis and immunoprecipitation
Identify receptor-ligand interactions by chemical crosslinking
“Fix” protein interactions to allow identification of weak or transient protein interactions
Identify near-neighbor protein interactions
Protein crosslinking to create bioconjugates via single-step reactions
Immobilize proteins onto amine-coated surfaces
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BS3, Bis（Sulfosuccinimidyl） Suberate原裝現貨 產品信息
