產(chǎn)品名稱:EGS
貨號:Pierce 21656
規(guī)格:1GM
Thermo Scientific Pierce EGS is a crosslinker that contains amine-reactive NHS-ester ends around a 12-atom spacer arm, which can be cleaved by treatment with hydroxylamine at pH 8.5.
Highlights:
Crosslinks formed are cleavable at pH 8.5 using hydroxylamine (Product #26103) for 3-6 hours at 37°C
Lactose dehydrogenase retained 60% of its activity after reversible crosslinking with EGS
Reaction conditions for water-soluble Sulfo-EGS are similar to EGS
Reactive groups: NHS esters (homobifunctional)
Reactive towards: amino groups
References:
Millar, J.B. and Rozengur, E. (1990). Chronic desensitization to bombesin by progressive down-regulation of bombesin receptors in Swiss 3T3 cells. J. Biol. Chem. 265, 12052-12058.
Browning, J. and Ribolini, A. (1989). Studies on the differing effects of tumor necrosis factor and lymphotoxin on the growth of several human tumor lines. J. Immunol. 143, 1859-1867. (EGS example)
名稱 | 廠商 | 貨號 | 規(guī)格 | 目錄價 |
EGS | Pierce | 21565 | 1 GM | 1871 |
BS3, Bis(Sulfosuccinimidyl) Suberate | Pierce | 21580 | 50 MG | 1005 |
DSS, 50MG | Pierce | 21655 | 50 MG | 610 |
Sulfo-SMCC | Pierce | 22322 | 50 MG | 1700 |
EDC | Pierce | 22980 | 5 GM | 958 |
EDC | Pierce | 22981 | 25 GM | 3052 |
Coomassie (Bradford) Assay Kit | Pierce | 23200 | 950 ML | 1065 |
BCA Protein Assay Reagent B | Pierce | 23224 | 25 ML | 443 |
BCA Protein Assay Kit/BCA蛋白濃度測定試劑盒 | Pierce | 23225 | KIT | 1843 |
BCA Protein Assay Kit/BCA蛋白濃度測定試劑盒 | Pierce | 23227 | KIT | 1137 |
BCA Protein Assay Reagent A | Pierce | 23228 | 500 ML | 874 |
Micro BCA Protein Assay Kit | Pierce | 23235 | 480 Assays | 1623 |