產品名稱：EZ-Link NHS-LC-LC-Biotin
貨號：Pierce 21343
規格：50 MG
Thermo Scientific EZ-Link NHS-LC-LC-Biotin is a doubly long-chain, NHS-ester activated biotinylation reagent for labeling primary amines （e.g., protein lysines）, whose membrane permeability enables it to be used for intracellular labeling.
This is one of three EZ-Link NHS-Biotin Reagents that enable simple and efficient biotin labeling of antibodies, proteins and any other primary amine-containing biomolecules in solution. Differing only in their spacer arm lengths, the three reagents offer researchers the possibility of optimizing labeling and detection experiments where steric hindrance of biotin binding is an important factor.
Product Details:
We manufacture biotinylation agents to the highest specifications to produce the most specific biotin labeled proteins, ensure the integrity of your data and to provide you with the highest degree of consistency. Each lot of a biotin labeling reagent is tested to meet our purity and solubility specifications.
NHS-activated biotins react efficiently with primary amine groups （-NH2） in pH 7-9 buffers to form stable amide bonds. Proteins, including antibodies, generally have abundant primary amines in the side chain of lysine （K） residues and the N-terminus of each polypeptide that are available as targets for labeling with NHS-activated biotin reagents. Several different NHS esters of biotin are available, with varying properties and spacer arm lengths. Non-sulfonated NHS-biotins require an organic solvent such as DMSO or DMF to be dissolved. The Sulfo-NHS ester biotins are water soluble, enabling reactions to be performed in the absence of organic solvents.
Highlights:
Lysine-reactive – label any antibody or other protein with biotin for use in protein methods
Irreversible – forms stable amide bonds that are permanent
Solubility – must be dissolved in DMSO or DMF before further dilution in aqueous buffers
Long reach – spacer arm （total length added to target） is 30.5 angstroms
Membrane-permeable – can be used to label inside cells （intracellular）
上海葉舟生物科技有限公司(上海浩然生物技術有限公司)在為生命科學領域提供豐富的產品與信息資源方面處于全國*地位，公司提供的產品涵蓋了二十多個國家近五十萬種產品，而且產品的數量與信息在不斷的增長和更新，公司提供的產品能夠使生命科學工作者加快對生物化學，分子生物學，細胞生物學以及蛋白質組學研究的認知，以及分子診斷和臨床醫學領域的應用。
為方便客戶檢索資料，公司建立了數據強大的網站:
為方便客戶與公司交流，公司設立了在線服 QQ:470003480 496799915
為方便客戶訂購產品，公司設立了直線電話: 021- 51870956 54046790
生物試劑
EZ-Link NHS-LC-LC-Biotin 原裝現貨 產品信息
