產(chǎn)品名稱：EZ-Link NHS-LC-Biotin
貨號(hào)：Pierce 21336
規(guī)格：50 MG
Thermo Scientific EZ-Link NHS-LC-Biotin is a long-chain, NHS-ester activated biotinylation reagent for labeling primary amines （e.g., protein lysines）, whose membrane permeability enables it to be used for general intracellular labeling.
This is one of three EZ-Link NHS-Biotin Reagents that enable simple and efficient biotin labeling of antibodies, proteins and any other primary amine-containing biomolecules in solution. Differing only in their spacer arm lengths, the three reagents offer researchers the possibility of optimizing labeling and detection experiments where steric hindrance of biotin binding is an important factor.
Highlights:
Lysine-reactive – label any antibody or other protein with biotin for use in protein methods
Irreversible – forms stable amide bonds that are permanent
Solubility – must be dissolved in DMSO or DMF before further dilution in aqueous buffers
Medium length – spacer arm （total length added to target） is 22.4 angstroms
Membrane-permeable – can be used to label inside cells （intracellular）
Product Details:
We manufacture biotinylation agents to the highest specifications to produce the most specific biotin labeled proteins, ensure the integrity of your data and to provide you with the highest degree of consistency. Each lot of a biotin labeling reagent is tested to meet our purity and solubility specifications.
NHS-activated biotins react efficiently with primary amine groups （-NH2） in pH 7-9 buffers to form stable amide bonds. Proteins, including antibodies, generally have abundant primary amines in the side chain of lysine （K） residues and the N-terminus of each polypeptide that are available as targets for labeling with NHS-activated biotin reagents. Several different NHS esters of biotin are available, with varying properties and spacer arm lengths. Non-sulfonated NHS-biotins require an organic solvent such as DMSO or DMF to be dissolved. The Sulfo-NHS ester biotins are water soluble, enabling reactions to be performed in the absence of organic solvents.