產(chǎn)品名稱:Streptavidin, Fluorescein (FITC) Conjugated
貨號(hào): Pierce 21224
規(guī)格: 1 MG
Applications for enzyme-labeled (HRP, AP) streptavidin:
Enzyme immunoassays (EIA), including enzyme-linked immunsorbent assays (ELISA) with biotinylated antibodies
Cell and tissue staining (for light microscopy)
Western blotting (WB) and other membrane-based biotin detection
Also see:
High Sensitivity
Streptavidin Conjugates
Much greater sensitivity and performance in many applications
Applications for fluorescent (FITC, TRITC or Texas Red* Fluor-labeled) streptavidin:
Immunofluorescent assays (IFA)
Tissue staining (for fluorescence microscopy)
Blot immunostaining
Cell staining (incl. fluorescent activated cell sorting)
Product Details:
Streptavidin is biotin-binding protein that was originally isolated from Streptomyces avidinii. In contrast to avidin, streptavidin has no carbohydrate and has a mildly acidic pI of 5. Thermo Scientific Pierce Streptavidin products use a recombinant form of streptavidin having a mass of 53,000 daltons and a near-neutral pI. Streptavidin is a tetrameric protein, with each subunit binding one molecule of biotin with affinity similar to that of avidin. Guanidinium chloride will dissociate avidin and streptavidin into subunits, but streptavidin is more resistant to dissociation.