Description
Application Designed for molecular weight determinations on silver stained SDS-PAGE （Laemmli） gels. The proteins supplied in these markers cover the range of molecular weights common to most proteins or their subunits. The markers are formulated to yield a distribution of well-defined bands of approximay equal intensity with low background after electrophoresis and staining using either ProteoSilver™ （PROTSIL1） or ProteoSilver™ Plus （PROTSIL2）.
Other Notes Will yield 9 bands with silver staining:
• α2-Macroglobulin, 180,000 Da
• β-Galactosidase, E. coli, 116,000 Da
• Phosphorylase b, Rabbit Muscle, 97,000 Da
• Serum Albumin, Bovine, 66,000 Da
• Fumarase, Porcine Heart, 48,500 Da
• Carbonic Anhydrase, Bovine Erythrocytes, 29,000 Da
• β-Lactoglobulin, Bovine Milk, 18,400 Da
• α-Lactalbumin, Bovine Milk, 14,200 Da
• Aprotinin, Bovine Lung, 6,500 Da
Packaging Vial contains 200 μl of protein markers. Supplied with one vial of 2× Laemmli sample buffer （S3401）.
Reconstitution Dilute the concentrate between 1:15 and 1:30 depending on the amount of protein loaded in the other lanes. Recommended loading volumes are 10 μl per well for mini gels （10 × 10 cm） or 20 μl per well for standard format gels （16 × 18 cm）.
Physical form Solution in 300 mM NaCl, 100 mM DTT, 3 mM NaN3, and 50% glycerol
Properties
form aqueous glycerol solution
usage sufficient for 300 standard gels （16 × 18 cm）
vial sufficient for 600 mini-gel applications
mol wt mol wt 6,500-180,000 Da
shipped in dry ice
storage temp. −20°C
Safety
Hazard Codes B
WGK Germany 1
References
reference Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685, （1970） Abstract
Heukeshoven, J. and Dernick, R., Simplified method for silver staining of proteins in polyacrylamide gels and the mechanism of silver staining. Electrophoresis 6, 103-112, （1985）
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