HA
Catalog Number: 28003
Gene Symbol: HA
Description: Anti-HA Mouse Monoclonal Antibody
Background: The HA tag is an eleven amino acid peptide derived from the influenza protein hemaglutinin (HA). This HA epitope tag is useful in
Western blotting and immunohistochemical localization of expressed fusion proteins when examined with antibodies raised specifically against the HA-epitope tag.
Source: HA is a mouse monoclonal antibody epitope mapping to an internal region of the infuenza hemaglutinin (HA) protein.
Application: Anti-HA monoclonal antibody is recommended for detection of proteins containing the HA tag by Western Blotting (starting dilution 1:200, dilution range 1:100-
1:1000), immunoprecipitation [1–2 μg per 100–500 μg of total protein (1 ml of cell lysate)] and immunofluorescence (starting dilution 1:50, dilution range 1:50-1:500).
Concentration: 1 mg/ml
Host: Mouse
Clonality: Monoclonal
Isotype: IgG
Purity: Purified from ascites
Format: Liquid
Storage buffer:
Preservative: no
Constituents: PBS (without Mg2+ ａnd Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Storage: Store at -20° C . Stable for one year from the date of shipment.
HA: NE-m18. Western blot analysis of whole cell lysates from MEF cells transfected with a plasmid encoding a HA-tagged protein (lane 1) and untransfected cells (lane 2).
HA: NE-m18. Immunofluorescence staining of methanol- fixed MEF cells showing the expression of HA-tagged proteins.
