AKT1(E49K) [26137]
[100ul]
| Catalog Number: 26137 |
| Gene Symbol: AKT1; AKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA |
| Description: Anti AKT1(E49K) Mouse Monoclonal Antibody |
| Background: AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinases, which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. Its function is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. There are evidences that mutation or abnormal expression in AKT1 is associated with cancers. |
| Immunogen: A synthetic peptide from the internal region of AKT1 which includes the mutation of E49K, human origin. |
| Applications: ELISA, WB, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:2000 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ ａnd Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes E49K mutant, but not wild-type AKT1 of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Download Datasheet: |
| Western blot: |
| Western blot analysis of recombinant AKT1(E49K) and wildtype proteins. Purified His-tagged AKT1(E49K) protein (lane 2) and corresponding wild type protein (lane 1) were blotted with Anti AKT1(E49K) monoclonal antibody (Cat. #26137). |
| Immunofluorescence: |
| Immunofluorescence of cells expressing AKT1 proteins with Anti AKT1(E49K) antibody. HEK293T cells were transfected with pCDNA3-GFP AKT1(WT) plasmid (left column) or pCDNA3-GFP AKT1(E49K) plasmid (right column), then fixed and stained with Anti AKT1(E49K) monoclonal antibody (Cat. #26137). |
| Related Products | |||
| Catalog# | Name | Size | Price |
| Anti AKT1 Rabbit Polyclonal Antibody | 100 μL | ￥2600 | |
| Anti AKT1(E49K) Mouse Monoclonal Antibody | 100 μL | ￥4800 | |
