因其所帶來(lái)的的峰對(duì)稱性、柱效和化學(xué)穩(wěn)定性，1.7 μm ACQUITY UPLC BEH C18和C8柱是廣泛適用于各種分析物通用的超高效色譜分離柱。因能在pH 1-12范圍內(nèi)操作，這些三鍵鍵合式烷基柱能夠利用寬pH調(diào)節(jié)對(duì)可離子化化合物的保留、選擇和靈敏度施加巨大影響，同時(shí)還能在低pH和高pH條件下保持優(yōu)異的柱穩(wěn)定性。
UPLC MS/MS assay for routine quantification of dabigatran – A direct thrombin inhibitor – In human plasma
This article described the development and full validation of rapid and accurate liquid chromatography method, coupled with tandem mass spectrometry detection, for quantification of dabigatran in human plasma with [ 13C 6]-dabigatran as internal standard. Plasma pretreatment involved a single step protein precipitation with methanol. Separation was performed by ultra performance reversed-phase chromatography on an Acquity UPLC BEH C8 100 mm × 1 mm × 1.7 μm column using a gradient elution mode. The phase was a mix of distilled water containing 0.1% formic acid and methanol containing 0.1% formic acid. Specific multiple reaction monitoring transitions were recorded in positive electrospray ionization. The method was linear over the concentration range of 2–500 μg/L. The intra- and inter-day precision values were below 11.3% and accuracy was within 93.8% and 108.8% for all QC levels (5, 75 and 400 μg/L). The lower limit of quantification was 2 μg/L. Total analysis time was to 10 min including sample preparation.
品名 | 編號(hào) |
ACQUITY UPLC BEH C8 Column, 130?, 1.7 µm, 2.1 mm X 150 mm, 1/pkg | 186003377 |
ACQUITY UPLC BEH C8 Column, 130?, 1.7 µm, 2.1 mm X 75 mm, 1/pkg | 186005606 |
ACQUITY UPLC BEH C8 Column, 130?, 1.7 µm, 1 mm X 50 mm, 1/pkg | 186002875 |
ACQUITY UPLC BEH C8 Column, 130?, 1.7 µm, 3 mm X 100 mm, 1/pkg | 186004665 |
ACQUITY UPLC BEH C8 Column, 130?, 1.7 µm, 2.1 mm X 100 mm, 1/pkg | 186002878 |
ACQUITY UPLC BEH C8 Column, 130?, 1.7 µm, 2.1 mm X 50 mm, 1/pkg | 186002877 |
ACQUITY UPLC BEH C8 Column, 130?, 1.7 µm, 1 mm X 100 mm, 1/pkg | 186002876 |
ACQUITY UPLC BEH C8 Column, 130?, 1.7 µm, 1 mm X 150 mm, 3/pkg | 176001046 |
ACQUITY UPLC BEH C8 Column, 130?, 1.7 µm, 2.1 mm X 30 mm, 3/pkg | 176001310 |
ACQUITY UPLC BEH C8 Column, 130?, 1.7 µm, 1 mm X 50 mm, 3/pkg | 176000882 |
ACQUITY UPLC BEH C8 Column, 130?, 1.7 µm, 1 mm X 100 mm, 3/pkg | 176000883 |
ACQUITY UPLC BEH C8 Column, 130?, 1.7 µm, 2.1 mm X 50 mm, 3/pkg | 176000884 |
ACQUITY UPLC BEH C8 Column, 130?, 1.7 µm, 2.1 mm X 100 mm, 3/pkg | 176000885 |
ACQUITY UPLC BEH C8 Column, 130?, 1.7 µm, 2.1 mm X 150 mm, 3/pkg | 176001050 |
ACQUITY UPLC BEH C8 Column, 130?, 1.7 µm, 3 mm X 100 mm, 3/pkg | 176001800 |
ACQUITY UPLC BEH C8 Column, 130?, 1.7 µm, 2.1 mm X 30 mm, 1/pkg | 186003910 |
ACQUITY UPLC BEH C8 Column, 130?, 1.7 µm, 3 mm X 50 mm, 1/pkg | 186004664 |
ACQUITY UPLC BEH C8 Column, 130?, 1.7 µm, 3 mm X 30 mm, 1/pkg | 186004663 |
ACQUITY UPLC BEH C8 Method Validation Kit, 130?, 1.7 µm, 2.1 mm X 150 mm, 3/pkg | 186006020 |
ACQUITY UPLC BEH C8 Method Validation Kit, 130?, 1.7 µm, 2.1 mm X 50 mm, 3/pkg | 186004046 |
ACQUITY UPLC BEH C8 Method Validation Kit, 130?, 1.7 µm, 2.1 mm X 100 mm, 3/pkg | 186004047 |
ACQUITY UPLC BEH C8 Column, 130?, 1.7 µm, 3 mm X 150 mm, 3/pkg | 176001801 |
ACQUITY UPLC BEH C8 Column, 130?, 1.7 µm, 3 mm X 75 mm, 1/pkg | 186005611 |
ACQUITY UPLC BEH C8 Column, 130?, 1.7 µm, 3 mm X 50 mm, 3/pkg | 176001799 |
ACQUITY UPLC BEH C8 Column, 130?, 1.7 µm, 3 mm X 30 mm, 3/pkg | 176001798 |
ACQUITY UPLC BEH C8 VanGuard Pre-column, 130?, 1.7 µm, 2.1 mm X 5 mm, 3/pkg | 186003978 |
ACQUITY UPLC BEH C8 Method Validation Kit, 130?, 1.7 µm, 3 mm X 100 mm, 3/pkg | 186004694 |
ACQUITY UPLC BEH C8 Method Validation Kit, 130?, 1.7 µm, 3 mm X 50 mm, 3/pkg | 186004693 |
