HEP-G2人肝癌細胞
細胞名稱:HEP-G2人肝癌細胞
細胞別稱:HEP-G2; Hep G2; HEP G2; HepG2; HEPG2
細胞簡稱:HepG2
產品貨號:TCH-C196
規格:1×10?cells/T25 培養瓶
種屬來源:人
組織來源:肝
疾病特征:肝癌
細胞形態:上皮細胞樣
生長特性:貼壁生長
培養體系:MEM+10%FBS+1%Glutamax+1%NEAA+1% Sodium Pyruvate+1%P/S
配套培養基貨號:TCH-G196
傳代比例:1:2-1:4,每2-3天換液一次
傳代周期:72-96 h
培養條件:氣相:95%空氣+5%CO 2,溫度:37℃
凍存條件:60%基礎培養基+30%FBS+10%DMSO,液氮儲存
質量檢測:細菌、真菌、支原體檢測均為陰性
細胞來源:HEP-G2人肝癌細胞來源于一名15歲的白人少年的肝癌組織。該細胞表達甲胎蛋白、白蛋白、α-2-巨球蛋白、α-1-抗、轉鐵蛋白、α-1-抗凝乳蛋白酶、結合珠蛋白、銅藍蛋白、纖溶酶原、補體C4、C3激活物、纖維蛋白原、α-1酸性糖蛋白、α-2-HS-糖蛋白、β-脂蛋白、結合蛋白;表達胰島素受體和胰島素樣生長因子IGFⅡ的受體;該細胞具有3-羥基-3-甲酰還原酶和肝甘油三酯脂肪酶的活性。目前尚未證明 HEP-G2人肝癌細胞中有HBV基因組。
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