JOE azide, 5-isomer|JOE N3,5-isomer
JOE is a fluorescent dye with the emission in yellow region of the spectrum. The fluorophore matches channel of HEX dye, another chlorinated fluorescein.
JOE dye is often used in qPCR; oligo labeling can be achieved using Click chemistry. This is an azide derivative of JOE for Click chemistry, pure 5-isomer.
General properties:
Appearance: | red solid |
Mass spec M+ increment: | 586.1 |
Molecular weight: | 587.37 |
Molecular formula: | C26H20N4Cl2O8 |
Solubility: | good in DMF, DMSO |
Quality control: | NMR 1H, HPLC-MS (95%) |
Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
JOE azide, 5-isomer|JOE N3,5-isomer
常用熒光染料的激發波長及發射波長
熒光染料 | Ex（nm） | Em（nm） |
FITC | 490 | 525 |
TRITC | 547 | 572 |
Cy2 | 489 | 506 |
Cy3 | 555 | 570 |
Cy5 | 646 | 620 |
Cy5.5 | 675 | 694 |
Cy7 | 750 | 773 |
Calcein | 494 | 517 |
Rhodamine 110 | 496 | 520 |
Rhodamine B | 555 | 580 |
5-Carboxy Fluorescein | 492 | 518 |
5-FAM, SE | 492 | 520 |
6-TAMRA SE | 543 | 575 |
6-Carboxy-X-rhodamine | 568 | 590 |
Alexa TM 488 | 490 | 520 |
BODIPY | 530 | 550 |
Alexa TM 546 | 555 | 570 |
Pyronin Y | 550 | 580 |
Thiadicarbocyanine | 651 | 670 |
Alexa TM 594 | 590 | 615 |
6-Aminofluorescein | 490 | 520 |
Dansyl chloride | 337 | 492 |
Rhodamine 123 | 507 | 529 |
4-Methylumbelliferone | 385 | 502 |
5-carboxy-2,7-dichlorofluorescein | 504 | 529 |
5-Carboxynapthofluorescein | 512 | 568 |
5-Carboxytetramethylrhodamine (5-TAMRA) | 542 | 568 |
5-FAM (5-Carboxyfluorescein) | 492 | 518 |
5-ROX (carboxy-X-rhodamine) | 567 | 591 |
5-TAMRA (5-Carboxytetramethylrhodamine) | 548 | 552 |
Acriflavin | 436 | 520 |
Alexa Fluor 350™ | 346 | 442 |
Alexa Fluor 430™ | 431 | 540 |
Alexa Fluor 488™ | 492 | 520 |
Alexa Fluor 532™ | 531 | 553 |
Alexa Fluor 568™ | 577 | 603 |
Alexa Fluor 633™ | 632 | 650 |
Alexa Fluor 680™ | 679 | 702 |
AMCA（Aminomethylcoumarin） | 345 | 425 |
Aminocoumarin | 346 | 442 |
Anthrocyl stearate | 381 | 446 |
APC-Cy7 | 655 | 755 |
Atabrine | 436 | 490 |
Aurophosphine | 490 | 515 |
Bisbenzamide | 360 | 461 |
Bodipy 492/515 | 490 | 515 |
Bodipy 500/510 | 500 | 510 |
Bodipy 530/550 | 528 | 547 |
Bodipy 576/589 | 579 | 590 |
Catecholamine | 410 | 470 |
Coumarin Phalloidin | 387 | 470 |
Dansyl Amine | 337 | 517 |
Dansyl Chloride | 372 | 518 |
Hydroxycoumarin | 325 | 386 |
NBD | 466 | 539 |
PerCP | 488 | 675 |
Nile Red | 556 | 590 |
Noradrenaline | 340 | 490 |
Thiolyte | 385 | 488 |
Merocyanin | 555 | 578 |
BODIPY TMR | 542 | 568 |
