FAM azide| FAM N3|FAM疊氮化物
FAM azide for Click chemistry labeling. FAM remains one of the most popular fluorescent lablels for various application. Most instruments capable of fluorescence detection, ranging from plate readers to fluorescence microscopes, are able to work in FAM channel.
With versatility of Click chemistry and this reagent, it is possible to attach this popular fluorophore to nearly any alkyne bearing molecule.
FAM azide is available both as solid compound, and as 10 mM solution in DMSO ready to use in our recommended labeling protocol. This product is a pure 5-isomer. FAM is a replacement for Alexa Fluor 488, DyLight 488.
FAM azide| FAM N3|FAM疊氮化物
General properties：
Appearance: | yellowish crystals |
Molecular weight: | 458.42 |
Molecular formula: | C24H18N4O6 |
Solubility: | soluble in polar organic solvents (DMF, DMSO, alcohols) |
Quality control: | NMR 1H, HPLC-MS (95%) |
Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
Spectral properties：
Excitation maximum, nm: | 494 |
ε, L⋅mol−1⋅cm−1: | 75000 |
Emission maximum, nm: | 520 |
Fluorescence quantum yield: | 0.9 |
CF260: | 0.20 |
CF280: | 0.17
|
熒光染料示例 | 激發光 |
Indo-1, Ca saturated | 331 nm |
Indo-1 Ca2+ | 346 nm |
Cascade Blue BSA pH 7.0 | 401 nm |
Cascade Blue | 398 nm |
LysoTracker Blue | 373 nm |
Alexa 405 | 401 nm |
LysoSensor Blue pH 5.0 | 374 nm |
LysoSensor Blue | 374 nm |
DyLight 405 | 399 nm |
DyLight 350 | 332 nm |
BFP (Blue Fluorescent Protein) | 380 nm |
Alexa 350 | 343 nm |
7-Amino-4-methylcoumarin pH 7.0 | 346 nm |
Amino Coumarin | 345 nm |
AMCA conjugate | 347 nm |
Coumarin | 360 nm |
7-Hydroxy-4-methylcoumarin | 360 nm |
7-Hydroxy-4-methylcoumarin pH 9.0 | 361 nm |
6,8-Difluoro-7-hydroxy-4-methylcoumarin pH 9.0 | 358 nm |
Hoechst 33342 | 352 nm |
Pacific Blue | 404 nm |
Hoechst 33258 | 352 nm |
Hoechst 33258-DNA | 352 nm |
Pacific Blue antibody conjugate pH 8.0 | 404 nm |
PO-PRO-1 | 434 nm |
PO-PRO-1-DNA | 435 nm |
POPO-1 | 433 nm |
POPO-1-DNA | 433 nm |
DAPI-DNA | 359 nm |
DAPI | 358 nm |
Marina Blue | 362 nm |
SYTOX Blue-DNA | 445 nm |
CFP (Cyan Fluorescent Protein) | 434 nm |
eCFP (Enhanced Cyan Fluorescent Protein) | 437 nm |
1-Anilinonaphthalene-8-sulfonic acid (1,8-ANS) | 375 nm |
Indo-1, Ca free | 346 nm |
1,8-ANS (1-Anilinonaphthalene-8-sulfonic acid) | 375 nm |
BO-PRO-1-DNA | 462 nm |
BOPRO-1 | 462 nm |
BOBO-1-DNA | 461 nm |
SYTO 45-DNA | 451 nm |
evoglow-Pp1 | 448 nm |
evoglow-Bs1 | 448 nm |
evoglow-Bs2 | 448 nm |
Auramine O | 431 nm |
DiO | 487 nm |
LysoSensor Green pH 5.0 | 447 nm |
Cy 2 | 489 nm |
LysoSensor Green | 447 nm |
Fura-2, high Ca | 336 nm |
Fura-2 Ca2+sup> | 336 nm |
SYTO 13-DNA | 488 nm |
YO-PRO-1-DNA | 491 nm |
YOYO-1-DNA | 491 nm |
eGFP (Enhanced Green Fluorescent Protein) | 488 nm |
LysoTracker Green | 503 nm |
GFP (S65T) | 489 nm |
BODIPY FL, MeOH | 502 nm |
Sapphire | 396 nm |
BODIPY FL conjugate | 503 nm |
MitoTracker Green | 490 nm |
MitoTracker Green FM, MeOH | 490 nm |
Fluorescein 0.1 M NaOH | 493 nm |
Calcein pH 9.0 | 494 nm |
Fluorescein pH 9.0 | 490 nm |
Calcein | 493 nm |
Fura-2, no Ca | 367 nm |
Fluo-4 | 494 nm |
FDA | 495 nm |
DTAF | 495 nm |
Fluorescein | 495 nm |
Fluorescein antibody conjugate pH 8.0 | 493 nm |
CFDA | 495 nm |
FITC | 495 nm |
Alexa Fluor 488 hydrazide-water | 493 nm |
DyLight 488 | 493 nm |
5-FAM pH 9.0 | 492 nm |
FITC antibody conjugate pH 8.0 | 495 nm |
Alexa 488 | 493 nm |
Rhodamine 110 | 497 nm |
Rhodamine 110 pH 7.0 | 497 nm |
Acridine Orange | 431 nm |
Alexa Fluor 488 antibody conjugate pH 8.0 | 499 nm |
BCECF pH 5.5 | 485 nm |
PicoGreendsDNA quantitation reagent | 502 nm |
SYBR Green I | 498 nm |
Rhodaminen Green pH 7.0 | 497 nm |
CyQUANT GR-DNA | 502 nm |
NeuroTrace 500/525, green fluorescent Nissl stain-RNA | 497 nm |
DansylCadaverine | 335 nm |
Rhodol Green antibody conjugate pH 8.0 | 499 nm |
Fluoro-Emerald | 495 nm |
Nissl | 497 nm |
Fluorescein dextran pH 8.0 | 501 nm |
Rhodamine Green | 497 nm |
5-(and-6)-Carboxy-2', 7'-dichlorofluorescein pH 9.0 | 504 nm |
DansylCadaverine, MeOH | 335 nm |
eYFP (Enhanced Yellow Fluorescent Protein) | 514 nm |
Oregon Green 488 | 498 nm |
Oregon Green 488 antibody conjugate pH 8.0 | 498 nm |
Fluo-3 | 506 nm |
BCECF pH 9.0 | 501 nm |
SBFI-Na+ | 336 nm |
Fluo-3 Ca2+ | 506 nm |
Rhodamine 123, MeOH | 507 nm |
FlAsH | 509 nm |
Calcium Green-1 Ca2+ | 506 nm |
Magnesium Green | 507 nm |
DM-NERF pH 4.0 | 493 nm |
Calcium Green | 506 nm |
Citrine | 515 nm |
LysoSensor Yellow pH 9.0 | 335 nm |
TO-PRO-1-DNA | 515 nm |
Magnesium Green Mg2+ | 507 nm |
Sodium Green Na+ | 507 nm |
TOTO-1-DNA | 514 nm |
Oregon Green 514 | 512 nm |
Oregon Green 514 antibody conjugate pH 8.0 | 513 nm |
NBD-X | 466 nm |
DM-NERF pH 7.0 | 509 nm |
NBD-X, MeOH | 467 nm |
CI-NERF pH 6.0 | 513 nm |
Alexa 430 | 431 nm |
Alexa Fluor 430 antibody conjugate pH 7.2 | 431 nm |
CI-NERF pH 2.5 | 504 nm |
Lucifer Yellow, CH | 428 nm |
LysoSensor Yellow pH 3.0 | 389 nm |
6-TET, SE pH 9.0 | 521 nm |
Eosin antibody conjugate pH 8.0 | 525 nm |
Eosin | 524 nm |
6-Carboxyrhodamine 6G pH 7.0 | 526 nm |
6-Carboxyrhodamine 6G, hydrochloride | 525 nm |
Bodipy R6G SE | 528 nm |
BODIPY R6G, MeOH | 528 nm |
6 JOE | 520 nm |
Cascade Yellow antibody conjugate pH 8.0 | 399 nm |
Cascade Yellow | 399 nm |
mBanana | 540 nm |
Alexa Fluor 532 antibody conjugate pH 7.2 | 528 nm |
Alexa 532 | 528 nm |
Erythrosin-5-isothiocyanate pH 9.0 | 533 nm |
6-HEX, SE pH 9.0 | 534 nm |
mOrange | 548 nm |
mHoneydew | 478 nm |
Cy 3 | 549 nm |
Rhodamine B | 543 nm |
DiI | 551 nm |
5-TAMRA-MeOH | 543 nm |
Alexa 555 | 553 nm |
Alexa Fluor 555 antibody conjugate pH 7.2 | 553 nm |
DyLight 549 | 555 nm |
BODIPY TMR-X, SE | 544 nm |
BODIPY TMR-X, MeOH | 544 nm |
PO-PRO-3-DNA | 539 nm |
PO-PRO-3 | 539 nm |
Rhodamine | 551 nm |
Bodipy TMR-X conjugate | 544 nm |
POPO-3 | 533 nm |
Alexa 546 | 562 nm |
BODIPY TMR-X antibody conjugate pH 7.2 | 544 nm |
Calcium Orange Ca2+ | 549 nm |
TRITC | 550 nm |
Calcium Orange | 549 nm |
Rhodaminephalloidin pH 7.0 | 558 nm |
MitoTracker Orange | 551 nm |
MitoTracker Orange, MeOH | 551 nm |
Phycoerythrin | 565 nm |
Magnesium Orange | 550 nm |
R-Phycoerythrin pH 7.5 | 565 nm |
5-TAMRA pH 7.0 | 553 nm |
5-TAMRA | 549 nm |
Rhod-2 | 552 nm |
FM 1-43 | 472 nm |
Rhod-2 Ca2+ | 553 nm |
Tetramethylrhodamine antibody conjugate pH 8.0 | 552 nm |
FM 1-43 lipid | 473 nm |
LOLO-1-DNA | 568 nm |
dTomato | 554 nm |
DsRed | 563 nm |
Dapoxyl (2-aminoethyl) sulfonamide | 372 nm |
Tetramethylrhodamine dextran pH 7.0 | 555 nm |
Fluor-Ruby | 554 nm |
Resorufin | 571 nm |
Resorufin pH 9.0 | 571 nm |
mTangerine | 568 nm |
LysoTracker Red | 578 nm |
Lissaminerhodamine | 572 nm |
Cy 3.5 | 578 nm |
Rhodamine Red-X antibody conjugate pH 8.0 | 573 nm |
Sulforhodamine 101, EtOH | 578 nm |
JC-1 pH 8.2 | 593 nm |
JC-1 | 592 nm |
mStrawberry | 575 nm |
MitoTracker Red | 578 nm |
MitoTracker Red, MeOH | 578 nm |
X-Rhod-1 Ca2+ | 580 nm |
Alexa Fluor 568 antibody conjugate pH 7.2 | 579 nm |
Alexa 568 | 576 nm |
5-ROX pH 7.0 | 578 nm |
5-ROX (5-Carboxy-X-rhodamine, triethylammonium salt) | 578 nm |
BO-PRO-3-DNA | 574 nm |
BOPRO-3 | 574 nm |
BOBO-3-DNA | 570 nm |
Ethidium Bromide | 524 nm |
ReAsH | 597 nm |
Calcium Crimson | 589 nm |
Calcium Crimson Ca2+ | 590 nm |
mRFP | 585 nm |
mCherry | 587 nm |
Texas Red-X antibody conjugate pH 7.2 | 596 nm |
HcRed | 590 nm |
DyLight 594 | 592 nm |
Ethidium homodimer-1-DNA | 528 nm |
Ethidiumhomodimer | 528 nm |
Propidium Iodide | 538 nm |
SYPRO Ruby | 467 nm |
Propidium Iodide-DNA | 538 nm |
Alexa 594 | 590 nm |
BODIPY TR-X, SE | 588 nm |
BODIPY TR-X, MeOH | 588 nm |
BODIPY TR-X phallacidin pH 7.0 | 590 nm |
Alexa Fluor 610 R-phycoerythrin streptavidin pH 7.2 | 567 nm |
YO-PRO-3-DNA | 613 nm |
Di-8 ANEPPS | 469 nm |
Di-8-ANEPPS-lipid | 469 nm |
YOYO-3-DNA | 612 nm |
Nile Red-lipid | 553 nm |
Nile Red | 559 nm |
DyLight 633 | 624 nm |
mPlum | 587 nm |
TO-PRO-3-DNA | 642 nm |
DDAO pH 9.0 | 648 nm |
Fura Red, high Ca | 434 nm |
Allophycocyanin pH 7.5 | 651 nm |
APC (allophycocyanin) | 650 nm |
Nile Blue, EtOH | 631 nm |
TOTO-3-DNA | 642 nm |
Cy 5 | 646 nm |
BODIPY 650/665-X, MeOH | 646 nm |
Alexa Fluor 647 R-phycoerythrin streptavidin pH 7.2 | 569 nm |
DyLight 649 | 652 nm |
Alexa Fluor 647 antibody conjugate pH 7.2 | 653 nm |
Alexa 647 | 653 nm |
Fura Red Ca2+ | 435 nm |
Atto 647 | 644 nm |
Fura Red, low Ca | 472 nm |
Carboxynaphthofluorescein pH 10.0 | 600 nm |
Alexa 660 | 664 nm |
Alexa Fluor 660 antibody conjugate pH 7.2 | 663 nm |
Cy 5.5 | 673 nm |
Alexa Fluor 680 antibody conjugate pH 7.2 | 679 nm |
Alexa 680 | 679 nm |
DyLight 680 | 678 nm |
Alexa Fluor 700 antibody conjugate pH 7.2 | 696 nm |
Alexa 700 | 696 nm |
FM 4-64, 2% CHAPS | 506 nm |
FM 4-64 | 508 nm |
