Anti-EPHB3/Eph receptor B3抗體,內皮細胞受體蛋白*激酶B3抗體
研究領域 神經生物學 信號轉導 激酶和磷酸酶
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep,
產品應用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (石蠟切片需做抗原修復)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 107kDa
細胞定位 細胞膜
性 狀 Lyophilized or Liquid
濃 度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human EPHB3/Eph receptor B3
亞 型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
訂購:或(成)客服
英文名稱 EPHB3/Eph receptor B3
抗體名稱 Anti-EPHB3/Eph receptor B3抗體,內皮細胞受體蛋白*激酶B3抗體
別 名 Cek10; EK2; Embryonic kinase 2; EPH Like Tyrosine Kinase 2; EPH-like kinase 2; ephb3; EPHB3_HUMAN; Ephrin receptor EphB3; Ephrin type B receptor 3; Ephrin type-B receptor 3; ETK2; hEK2; Human Embryo Kinase 2; Mdk5; Sek4; TYRO6; Tyrosine protein kinase receptor HEK2; Tyrosine protein kinase TYRO6; Tyrosine-protein kinase TYRO6.
濃 度 1mg/1ml
規 格 0.1ml/0.2ml(100ug/200ug)
公司提供的抗體達一萬多種,公司擁有Complexin I/復合素1抗體一抗,二抗,標記一抗,標記二抗等抗體種類,*價供應。
多克隆抗體制備服務:應用保證型抗體制備(ELISA,Western Blot,IHC)和修飾特異性抗體制備(磷酸化,甲基化,乙酰化)。
單克隆抗體制備服務:應用保證型抗體制備(ELISA,Western Blot,IHC)和修飾特異性抗體制備(磷酸化,甲基化,乙酰化)。
抗體純化服務:Protein A/G/L純化,抗原親和純化。
zui終抗體質檢:成品抗體或抗血清,必須通過客戶要求的所有驗證(ELISA,Western Blot,IHC等)。所有的抗原和抗體驗證數據提交客戶。
免疫組化的呈色深淺可反映抗原存在的數量,可作為定性、定位和定量的依據。
1.陽性細胞染色分布有三種類型:細胞漿、細胞核、細胞膜表面。大部分抗原見于細胞漿,可見于整個胞漿或部分胞漿
2.陽性細胞分布可分為灶型和彌漫性
3.由于細胞內含抗原量不同,所以染色強度不一。如果細胞之間染色強度相同,常提示其反應為非特異性
4.陽性細胞染色定位于單個細胞,且與陰性細胞相互交雜分布;而非特異性染色常不限于單個細胞,而是累及一片細胞。
5.切片邊緣、刀痕或褶皺區域,壞死或擠壓的細胞區,膠原結締組織等,常表現為相同的陽性染色強度,不能用于判斷陽性。
產品介紹 The Eph subfamily represents the largest group of receptor protein tyrosine kinases identified to date (1–3). While the biological activities of these receptors have yet to be determined, there is increasing evidence that they are involved in central nervous system function and in development (1–3). The Eph subfamily receptors of human origin (and their murine/avian homologs) include EphA1 (Eph), EphA2 (Eck), EphA3 (Hek4), EphA4 (Hek8), EphA5 (Hek7), EphA6 (Hek12), EphA7 (Hek11/MDK1), EphA8 (Hek3), EphB1 (Hek6), EphB2 (Hek5), EphB3 (Cek10, Hek2), EphB4 (Htk), EphB5 (Hek9) and EphB6 (Mep). Ligands for Eph receptors include ephrin-A4 (LERK-4) which binds EphA3 and EphB1. In addition, ephrin-A2 (ELF-1) has been described as the ligand for EphA4, ephrin-A3 (Ehk1-L) as the ligand for EphA5 and ephrin-B2 (Htk-L) as the ligand for EphB4 (Htk) (4–7).
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