上海研盟生物科技有限公司PD-1抗體-程序性死亡1抗體*,主要應用于WB、IHC、IF、ELISA、流式細胞術等實驗中。說明書隨貨發送,您也可以直接我司在線客服索取。客服
簡單介紹:
中文名稱:程序性死亡1抗體
英文名稱:Anti-PD-1
產品編號:BYK-1867R
產品別名:CD279; CD279 antigen; hPD 1; hPD l; hPD-1; hSLE1; PD 1; PD-1; PD1; PDCD 1; PDCD1; PDCD1_HUMAN; Programmed cell death 1; Programmed cell death protein 1; Protein PD 1; Protein PD-1; SLEB2; Systemic lupus erythematosus susceptibility 2.
產品規格:0.1ml、0.2ml、1ml
產品用途:科研實驗
產品價格:請報價
說明書:請添加客服直接索取
抗體來源:該指標有兩種產品,一種是兔來源抗體,一種是鼠來源抗體
克隆類型:兔來源為多克隆抗體,鼠來源單克隆抗體
交叉反應:請索取說明書查看
性 狀:Lyophilized or Liquid
濃 度:1mg/1ml
亞 型:IgG
純化方法: affinity purified by Protein A
儲 存 液: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol
保存條件: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PD-1抗體-程序性死亡1抗體產品介紹:This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases.
CD279 is an immunoglobulin superfamily member, also known as Programmed Cell Death 1. Programmed Cell Death 1 is expressed on a subset of CD4-CD8 thymocytes, and on activated T and B cells. Programmed Cell Death 1 is thought to be involved in lymphocyte clonal selection and peripheral tolerance. The Programmed Cell Death 1 ligands, PDL1 (also known as B7H1) and PDL2 (B7DC), are members of the B7 immunoglobulin superfamily.
多肽的溶解與保存
一、凍干多肽的溶解
1、多肽溶解的主要問題是二級結構的形成。雖然疏水肽鏈二級結構的形成更明顯,但除zui短的肽鏈外,此現象產生在幾乎所有肽鏈,與極性無關。所以,溶解多肽的*個原則是使用無菌蒸餾水或去離子水,當然無氧水。多肽溶液可能遇到細菌降解,為防止此情況的發生,應溶解在無菌的蒸餾水中或用0.45或0.2孔徑的濾膜過濾除菌。含有Cys、Met、Trp的多肽很容易氧化,應溶于無氧的水中,無氧水可通過注入惰性氣體(氮、氦、氬)減壓除氣得到;
2、若多肽不溶于純水,超聲處理有助于打碎顆粒并增加溶解度。注意:超聲處理會引起溶液發熱或多肽降解;
3、若多肽含有多個堿性氨基酸,使用(1-10%)的乙酸水溶液:對于疏水性強的多肽,應使用50%的乙酸;
4、若多肽中含有大量酸性氨基酸,可用氨溶液(1-10%)或乙酸乙基*或重碳酸鹽等揮發性的堿性緩沖液溶解。pH值在層析前必須調整;
5、異丙醇和乙腈能溶解中等大小的多肽。若肽要上柱,有機溶劑的量必須很少否則將嚴重影響停留時間;
6、若多肽因含有Val、Leu、Met、Phe、Tyr、Ala等芳香鏈而高度疏水、或為中性肽時,DMF或DMSO等膜變性劑的使用,有利于多肽的溶解:
a.高濃度模變性劑通過破壞多肽的二級結構而助溶;
b.膜變性劑適于多肽分析液的制備,但可能會對其生物活性的研究工作造成干擾;
c.DMF是變性劑(zui高濃度可達30%),滴加至多肽溶解;
d.反相層析法時,DMF將與洗脫液前鋒一起流出,根據入量的多少,峰值可能很高。大多數肽能在大量DMF流出后幾分鐘內流出,若肽鏈很小,洗脫太早,多肽的量將很低。
相關產品:
鈣粘蛋白相關的神經受體1(CNR-1)ELISA試劑盒,96T/48T科研實驗
*加壓素(AVP)ELISA試劑盒,96T/48T科研實驗
毛細血管擴張性共濟失調突變基因(ATM)ELISA試劑盒,96T/48T科研實驗
酸性神經鞘磷脂酶(ASM)ELISA試劑盒,96T/48T科研實驗
花生四烯酸5脂加氧酶(ALOX-5)ELISA試劑盒,96T/48T科研實驗
5脂加氧酶(5-LO/LOX)ELISA試劑盒,96T/48T科研實驗
芳香烴受體(AhR)ELISA試劑盒,96T/48T科研實驗
晚期糖基化終末產物受體(RAGE/AGER)ELISA試劑盒,96T/48T科研實驗
特異性巨噬細胞武裝因子(SMAF)ELISA試劑盒,96T/48T科研實驗
促有絲分裂因子(MF/MPF)ELISA試劑盒,96T/48T科研實驗
腺苷酸環化酶1(AC-1)ELISA試劑盒,96T/48T科研實驗
可溶性腺苷酸環化酶(sAC)ELISA試劑盒,96T/48T科研實驗
抗鼠抗體(HAMA)ELISA試劑盒,96T/48T科研實驗
鈣離子通道抗體(VGCC)ELISA試劑盒,96T/48T科研實驗
溴脫氧核苷*(BrdU)ELISA試劑盒,96T/48T科研實驗
胃粘液素(GM)ELISA試劑盒,96T/48T科研實驗
纖維母細胞表面抗原(FSP)ELISA試劑盒,96T/48T科研實驗
轉移因子(TF)ELISA試劑盒,96T/48T科研實驗
*脫羧酶(ODC)ELISA試劑盒,96T/48T科研實驗
黑色素瘤相關抗原(MAGE)ELISA試劑盒,96T/48T科研實驗
前膠原C端肽酶(PCP)ELISA試劑盒,96T/48T科研實驗
多形核白細胞彈性蛋白酶(PMN Elastase)ELISA試劑盒,96T/48T科研實驗
補體片斷3b(C3b)ELISA試劑盒,96T/48T科研實驗
補體片斷4b(C4b)ELISA試劑盒,96T/48T科研實驗