中文名稱：整合素αVβ5抗體
英文名稱：Anti-Integrin Alpha V + Beta 5
產(chǎn)品編號(hào)：BYK-1356R
別名：alpha 5; alpha polypeptide; CD49e; CD51; fibronectin receptor; FNRA; Integrin alpha V; Integrin beta 5; ITGA5; ITGAV; ITGB5; VLA5A; VNRA.
產(chǎn)品介紹：Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metatastatic diffusion of tumour cells. ITAGV encodes integrin alpha chain V. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing integrin alpha V undergoes post-translational cleavage to yield disulfide-linked heavy and light chains, that combine with multiple integrin beta chains to form different integrins. Among the known associating beta chains (beta chains 1,3,5,6, and 8; ITGB1, ITGB3, ITGB5, ITGB6, and ITGB8), each can interact with extracellular matrix ligands; the alpha V beta 3 integrin, perhaps the most studied of these, is referred to as the Vitronectin receptor (VNR). In addition to adhesion, many integrins are known to facilitate signal transduction.
此產(chǎn)品本公司有標(biāo)記的一抗出售，相關(guān)標(biāo)記有：Alexa Fluor 350 標(biāo)記、Alexa Fluor 488 標(biāo)記、Alexa Fluor 555 標(biāo)記、Alexa Fluor 647 標(biāo)記、AP標(biāo)記、APC標(biāo)記、Biotin標(biāo)記、Cy3標(biāo)記、Cy5標(biāo)記、Cy5.5標(biāo)記、Cy7標(biāo)記、FITC標(biāo)記、Gold標(biāo)記、HRP標(biāo)記、PE標(biāo)記、PE-Cy3標(biāo)記、PE-CY5標(biāo)記、PE-CY5.5標(biāo)記、PE-CY7標(biāo)記、RBITC標(biāo)記
規(guī)格：0.1ml/0.2ml
濃度：1mg/1ml
抗體來源：本公司抗體有兔來源和鼠來源等
克隆類型：兔來源為多抗，鼠來源為單抗
產(chǎn)品類型：一抗
Integrin Alpha V + Beta 5抗體-整合素αVβ5抗體保存條件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
避免反復(fù)凍融，保質(zhì)期一年。
Integrin Alpha V + Beta 5抗體-整合素αVβ5抗體免疫組化問題解答
1、染色過強(qiáng)？
原 因：抗體濃度過高或孵育時(shí)間過長(zhǎng)
解決方法：降低抗體滴度、抗體孵育時(shí)間：室溫1小時(shí)或4度過夜
原 因：孵育溫度過高超過37度
解決方法：一般在室溫20-28度
原 因：DAB顯色時(shí)間過長(zhǎng)或濃度過高
解決方法：顯色時(shí)間不超過5-12分鐘，以顯微鏡下觀察為準(zhǔn)
2、非特異性背景染色？
原 因：操作過程中沖洗不充分
解決方法：每步?jīng)_洗3次每次5分鐘
原 因：組織中含過氧化物酶未阻斷
解決方法：可再配置新鮮3%H2O2封閉孵育時(shí)間延長(zhǎng)
原 因：組織中含內(nèi)原性*
解決方法：正常非免疫動(dòng)物血清再封閉
原 因：血清蛋白封閉不充分
解決方法：血清蛋白封閉不充分
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