上海研盟生物科技有限公司Anti-Integrin Alpha V+Beta 3抗體,整合素αVβ3抗體*,主要應用于WB、IHC、IF、ELISA、流式細胞術等實驗中。說明書隨貨發送,您也可以直接我司在線客服索取。:
中文名稱:整合素αVβ3抗體
英文名稱:Anti-Integrin Alpha V+Beta 3(CD51+CD61)抗體
產品編號:BYK-1310R
別名:CD51 + CD61; CD51; CD61; GP3A; GPIIIa; integrin alpha v; integrin beta 3; intregrin alpha v beta 3; ITGAV; ITGB3; Plaet membrane glycoprotein IIIa; Vitronectin receptor alpha subunit; VNRA; Antigen identified by monoclonal antibody L230; CD51 antigen; CD61 antigen; DKFZp686A08142; Integrin alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); integrin alpha v; Integrin beta 3 (plaet glycoprotein IIIa, antigen CD61); Integrin beta chain beta 3; intregrin alpha v beta 3; ITGAV; MSK8; Plaet glycoprotein IIIa; Plaet membrane glycoprotein IIIa; Vitronectin receptor alpha subunit; Vitronectin receptor subunit alpha.
此產品本公司有標記的一抗出售,相關標記有:Alexa Fluor 350 標記、Alexa Fluor 488 標記、Alexa Fluor 555 標記、Alexa Fluor 647 標記、AP標記、APC標記、Biotin標記、Cy3標記、Cy5標記、Cy5.5標記、Cy7標記、FITC標記、Gold標記、HRP標記、PE標記、PE-Cy3標記、PE-CY5標記、PE-CY5.5標記、PE-CY7標記、RBITC標記
規格:0.1ml/0.2ml
濃度:1mg/1ml
抗體來源:本公司抗體有兔來源和鼠來源等
克隆類型:兔來源為多抗,鼠來源為單抗
產品類型:一抗
Anti-Integrin Alpha V+Beta 3抗體,整合素αVβ3抗體產品介紹:Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. They are known to participate in cell adhesion as well as cell-surface mediated signalling. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain.
CD51 encodes integrin alpha chain V. The I-domain containing integrin alpha V undergoes post-translational cleavage to yield disulfide-linked heavy and light chains, that combine with multiple integrin beta chains to form different integrins. The CD61 protein product is the integrin beta chain beta 3. Integrin beta 3 is found along with the alpha IIb chain in plaets.
Integrin alpha V/beta 3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase 2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha V/beta 3 recognizes the sequence R-G-D in a wide array of ligands. The alpha V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase 2, osteopontin, osteomodulin, prothrombin, thrombospondin and von Willebrand factor. They recognize the sequence R-G-D in a wide array of ligands.
保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.避免反復凍融,保質期一年。
上海研盟生物科技有限公司多抗親和層析純化
多抗的純化是一件比較簡單的事情,因為人工干預比較大,所以對實驗者的操作依賴性較強。很多新手覺得這是一件比較復雜困難的工作,但是如果理解了原理,就可以自行改進實驗了。多抗純化方法比較多,過去有用鹽析法純化的,有用辛酸-硫酸銨沉淀的方式純化的,也有用冷酒精沉淀的方式純化的,也有后來的離子交換層析和Protein A、G、A/G 純化的,但是在今天,市場上幾乎所有的抗體都是通過抗原親和純化得到的,與其它方法相比,抗原親和層析得到的抗體效率高、產量高、產品純、操作簡單。鑒于很多網友問起這方面的問題,我就詳細地講一講親和純化原理和操作。
說到親和層析,還得從上個世紀60年代說起。當年美國NIH(National Institutes of Health)
有個學生在研究一種酶和抑制劑之間的相互作用的時候,突發奇想:既然此酶可以和抑制劑結合,那如果把抑制劑固定起來,不就可以用來純化酶了嗎?試了一下覺得果然可行于是發了論文,從那之后,就出現了各種各樣的親和層析,親和配基與配體可以是酶與底物、酶與抑制劑、激素與受體、抗原和抗體、糖蛋白與凝集素、*與親和素、*與二價金屬離子、卟啉環與亞鐵離子等。親和層析精髓:將一種分子固定,用來吸附另一種特異性分子,zui后將其洗脫下來。
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