昆蟲端粒酶ELISA試劑盒●產品規格:96T(人份)/48T(人份)
單孔檢測:96T,可以檢測90份樣本,留5個孔做標準曲線,一個孔做空白對照;
48T,可以檢測42份樣本,留5個孔做標準曲線,一個孔做空白對照;
●品牌:子科生物ZIKER,美國R&D,美國immonoway,美國sciencell,德國IBL
●適用范圍:實驗結果僅供科研參考,不推薦用于臨床診斷結果。
昆蟲端粒酶ELISA試劑盒的基本原理
就是將抗原抗體固定到特定的載體上進行反應,并通過酶催化底物發生化學變化將抗原抗體反應通過顏色的形式顯現出來。
(1)將抗原抗體反應固相化:即試驗中的包被環節,使抗原或抗體吸附于固相載體表面。其機制可能是聚苯乙烯表面間的疏水基團可以無選擇性地吸附蛋白分子。此物理性吸附不破壞蛋白分子結構,保持其生物學特性和免疫學活性;
(2)封閉:固相吸附蛋白是非特異性的,在包被目的免疫蛋白后,未吸附蛋白的固相表面仍然有吸附其它蛋白的能力,為了保障抗原抗體反應的特異性,因此,包被剩余的固相表面應該用抗原抗體反應無關蛋白封閉。一般認為牛血清白蛋白是zui為理想的封閉劑,也可用脫脂奶粉、明膠、牛血清替代。
(3)抗原抗體反應:抗原或抗體在適當的介質下可進行特異的抗原抗體反應。 (4)酶反應:酶可以通過共價鍵與抗原或抗體連接形成結合物,當抗原抗體反應的時候,被檢測靶目標中也結合了酶分子。
(5)ELISA試劑盒底物反應:結合在抗原抗體中的酶分子,可以促使底物發生顏色反應,檢測人員可以裸眼觀察,可以通過顏色來判定是否有免疫反應的存在,通過顏色的深淺判斷標本中相應抗原或抗體的含量,也可借用酶標儀在適當的波長讀取吸光度值。
用于標記ELISA試劑盒里抗體或抗抗體的酶須具有下列特性:
1、有高度的活性和敏感性;
2、在室溫下穩定;
3、反應產物易于顯現;
4、能商品化生產。
當前應用較多的有辣根過氧化物酶(HRP)、堿性磷酸酶、葡萄糖氧化酶等,其中以HRP應用zui廣。
結果判斷與分析:
1、 儀器值:于波長450nm的酶標儀上讀取各孔的OD值
2、 以吸光度OD值為縱坐標(Y),相應的LTB4標準品濃度為橫坐標(X),做得相應的曲線,樣品的LTB4含量可根據其OD值由標準曲線換算出相應的濃度,再乘以稀釋倍數;或用標準物的濃度與OD值計算出標準曲線的回歸方程式,將樣品的OD值代入方程式,計算出樣品濃度,再乘以稀釋倍數,即為樣品的實際濃度。
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