產(chǎn)品名稱:Anti-OPRS1抗體,衰老相關(guān)Sigma受體蛋白抗體
產(chǎn)品編號:BYK-5111R
英文名稱:Anti-OPRS1
中文名稱:衰老相關(guān)Sigma受體蛋白抗體
英文別名:Sig-1R; sigma-1R; Aging associated gene 8 protein; AL024364; hSigmaR1; mSigmaR1; opioid receptor, sigma 1; opioid receptor, sigma 1 isoform 1; RP23 167I12.6; SIG 1R; sigma1 receptor; Sigma1R; SIGMAR1; SR BP; SR-BP; SR31747 binding protein; SRBP; SGMR1_HUMAN; Sigma non-opioid intracellular receptor 1; Aging-associated gene 8 protein; SR31747-binding protein; Sigma 1-type opioid receptor; SIG-1R; hSigmaR1; AAG8; SIGMAR1.
產(chǎn)品規(guī)格:0.1ml/0.2ml
此抗體本公司有單抗和多抗,可用于免疫組化(IHC),免疫印跡(WB),酶聯(lián)免疫(ELISA),IF、IP、流式細(xì)胞術(shù)等實驗(可來電索取說明)
保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Anti-OPRS1抗體,衰老相關(guān)Sigma受體蛋白抗體
英文介紹:This gene encodes a receptor protein that interacts with a variety of psychotomimetic drugs, including cocaine and amphetamines. The receptor is believed to play an important role in the cellular functions of various tissues associated with the endocrine, immune, and nervous systems. As indicated by its previous name, opioid receptor sigma 1 (OPRS1), the product of this gene was erroneously thought to function as an opioid receptor; it is now thought to be a non-opioid receptor. Mutations in this gene has been associated with juvenile amyotrophic lateral sclerosis 16. Alternative splicing of this gene results in transcript variants encoding distinct isoforms.
此產(chǎn)品本公司有標(biāo)記的一抗出售,相關(guān)標(biāo)記有:Alexa Fluor 350 標(biāo)記、Alexa Fluor 488 標(biāo)記、Alexa Fluor 555 標(biāo)記、Alexa Fluor 647 標(biāo)記、AP標(biāo)記、APC標(biāo)記、Biotin標(biāo)記、Cy3標(biāo)記、Cy5標(biāo)記、Cy5.5標(biāo)記、Cy7標(biāo)記、FITC標(biāo)記、Gold標(biāo)記、HRP標(biāo)記、PE標(biāo)記、PE-Cy3標(biāo)記、PE-CY5標(biāo)記、PE-CY5.5標(biāo)記、PE-CY7標(biāo)記、RBITC標(biāo)記
注意事項:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Anti-OPRS1抗體,衰老相關(guān)Sigma受體蛋白抗體轉(zhuǎn)膜(Transfer)
我們*在Western實驗中選用PVDF膜。硝酸纖維素膜(NC膜)或PVDF膜(二氟化樹脂膜膜孔徑:0.45um)也可以使用,但硝酸纖維素膜比較脆,在操作過程中特別是用鑷子夾取等過程中容易裂開。膜的使用請參考生產(chǎn)商的*使用步驟。
通常如果使用Bio-Rad的標(biāo)準(zhǔn)濕式轉(zhuǎn)膜裝置,可以設(shè)定轉(zhuǎn)膜電壓120V,轉(zhuǎn)膜時間為30-60分鐘。具體的轉(zhuǎn)膜時間要根據(jù)目的蛋白的大小而定,目的蛋白的分子量越大,需要的轉(zhuǎn)膜時間越長,目的蛋白的分子量越小,需要的轉(zhuǎn)膜時間越短。
在轉(zhuǎn)膜過程中,特別是高電流快速轉(zhuǎn)膜時,通常會有非常嚴(yán)重的發(fā)熱現(xiàn)象,把轉(zhuǎn)膜槽放置在冰浴中進行轉(zhuǎn)膜。轉(zhuǎn)膜的效果可以觀察所使用的預(yù)染蛋白質(zhì)分子量標(biāo)準(zhǔn),通常分子量zui大的1-2條帶較難全部轉(zhuǎn)到膜上。轉(zhuǎn)膜的效果也可以用麗春紅染色液對膜進行染色,以觀察實際的轉(zhuǎn)膜效果。也可以用考馬斯亮藍(lán)快速染色液對完成轉(zhuǎn)膜的SDS-PAGE膠進行染色,以觀察蛋白的殘留情況。
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