產(chǎn)品名稱：Anti-Epsin 2抗體,內(nèi)吞作用輔助蛋白EPN2抗體

產(chǎn)品編號：BYK-13102R
英文名稱：Anti-Epsin 2
中文名稱：內(nèi)吞作用輔助蛋白EPN2抗體
英文別名：EHB21; EPN 2; EPN-2; Epn2; EPN2_HUMAN; EPS 15 interacting protein 2; EPS-15-interacting protein 2; Eps15 binding protein; Epsin 2; Epsin-2; Epsin2; KIAA1065; OTTHUMP00000065808; OTTHUMP00000065809; OTTHUMP00000065810; OTTHUMP00000065811; OTTHUMP00000065889.
產(chǎn)品規(guī)格：0.1ml/0.2ml
此抗體本公司有單抗和多抗，可用于免疫組化（IHC），免疫印跡（WB），酶聯(lián)免疫(ELISA)，IF、IP、流式細(xì)胞術(shù)等實(shí)驗(yàn)（可來電索取說明）
保存條件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Anti-Epsin 2抗體,內(nèi)吞作用輔助蛋白EPN2抗體產(chǎn)品現(xiàn)貨銷售，其他同類產(chǎn)品：

byk-1046R    巨噬細(xì)胞炎癥因子1β 抗體 MIP-1β    Anti-MIP-1 Beta/CCL4
byk-11046R    神經(jīng)細(xì)胞粘附分子CALL抗體    Anti-CHL1/CALL
byk-1045R    巨噬細(xì)胞炎癥因子1α抗體 MIP-1α    Anti-MIP-1 Alpha/CCL3
byk-11045R    補(bǔ)體組分1q子成分樣蛋白1抗體    Anti-C1QL1
byk-1044R    巨噬細(xì)胞移動抑制因子抗體    Anti-MIF
byk-11044R    磷酸化信號轉(zhuǎn)導(dǎo)功能磷蛋白DAB2抗體    Anti-phospho-DAB2 (Ser24)
byk-1633R    肌球蛋白輕鏈激酶抗體    Anti-MLCK 
byk-11633R    磷酸化*受體1抗體    Anti-phospho-GluR1(Ser845)
byk-1849R    中期因子/肝素結(jié)合生長因子抗體    Anti-Midkine
byk-11849R    腸和大腦特定同源蛋白2抗體    Anti-Gbx2
byk-0762R    中腦核仁蛋白抗體    Anti-Midnolin
byk-1849R    中期因子/肝素結(jié)合生長因子抗體    Anti-Midkine
byk-11849R    腸和大腦特定同源蛋白2抗體    Anti-Gbx2
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byk-0909R    蜜蜂微孢子蟲蛋白抗體    Anti-Microsporidia protien
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byk-11412R    松弛素3抗體    Anti-Relaxin 3
byk-1412R    腫瘤轉(zhuǎn)移相關(guān)蛋白1抗體    Anti-MTA1
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Anti-Epsin 2抗體,內(nèi)吞作用輔助蛋白EPN2抗體轉(zhuǎn)膜(Transfer):

我們*在Western實(shí)驗(yàn)中選用PVDF膜。硝酸纖維素膜(NC膜)或PVDF膜（二氟化樹脂膜膜孔徑：0.45um)也可以使用，但硝酸纖維素膜比較脆，在操作過程中特別是用鑷子夾取等過程中容易裂開。膜的使用請參考生產(chǎn)商的*使用步驟。
通常如果使用Bio-Rad的標(biāo)準(zhǔn)濕式轉(zhuǎn)膜裝置，可以設(shè)定轉(zhuǎn)膜電壓120V，轉(zhuǎn)膜時(shí)間為30-60分鐘。具體的轉(zhuǎn)膜時(shí)間要根據(jù)目的蛋白的大小而定，目的蛋白的分子量越大，需要的轉(zhuǎn)膜時(shí)間越長，目的蛋白的分子量越小，需要的轉(zhuǎn)膜時(shí)間越短。
在轉(zhuǎn)膜過程中，特別是高電流快速轉(zhuǎn)膜時(shí)，通常會有非常嚴(yán)重的發(fā)熱現(xiàn)象，把轉(zhuǎn)膜槽放置在冰浴中進(jìn)行轉(zhuǎn)膜。轉(zhuǎn)膜的效果可以觀察所使用的預(yù)染蛋白質(zhì)分子量標(biāo)準(zhǔn)，通常分子量zui大的1-2條帶較難全部轉(zhuǎn)到膜上。轉(zhuǎn)膜的效果也可以用麗春紅染色液對膜進(jìn)行染色，以觀察實(shí)際的轉(zhuǎn)膜效果。也可以用考馬斯亮藍(lán)快速染色液對完成轉(zhuǎn)膜的SDS-PAGE膠進(jìn)行染色，以觀察蛋白的殘留情況。

Anti-Epsin 2抗體,內(nèi)吞作用輔助蛋白EPN2抗體

英文介紹：Elucidation of the mechanism by which receptor tyrosine kinases (RTKs) modulate cellular physiology in response to stimuli is critical to the understanding of growth regulation. Miscues in RTK signaling pathways can result in cellular transformation and ultimay in cancer. Two novel EGF receptor substrates have been described, designated EGF-receptor pathway substrates 8 and 15, or Eps8 and Eps15. Epsin is a binding partner to Eps15. Both epsin and Eps15 have an ubiquitous tissue distribution but are concentrated in presynaptic nerve terminals specialized for the Clathrin-mediated endocytosis of synaptic vesicles. Disruption of epsin function blocks Clathrin-mediated endocytosis. Epsin, along with its binding partner Eps15, is proposed to be involved in the assistance of Clathrin coat rearrangement during Clathrin coated pit invagination. Epsin 2a, and 2b are splicing variants of epsin 2, which is associated with Clathrin-mediated endocytosis and are enriched in the brain in the peri-Golgi region.
此產(chǎn)品本公司有標(biāo)記的一抗出售，相關(guān)標(biāo)記有：Alexa Fluor 350 標(biāo)記、Alexa Fluor 488 標(biāo)記、Alexa Fluor 555 標(biāo)記、Alexa Fluor 647 標(biāo)記、AP標(biāo)記、APC標(biāo)記、Biotin標(biāo)記、Cy3標(biāo)記、Cy5標(biāo)記、Cy5.5標(biāo)記、Cy7標(biāo)記、FITC標(biāo)記、Gold標(biāo)記、HRP標(biāo)記、PE標(biāo)記、PE-Cy3標(biāo)記、PE-CY5標(biāo)記、PE-CY5.5標(biāo)記、PE-CY7標(biāo)記、RBITC標(biāo)記
注意事項(xiàng):This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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