Anti-Von Willebrand Factor抗體,血管假性血友病因子/血管性血友病因子抗體產(chǎn)品現(xiàn)貨銷售,其他同類產(chǎn)品:
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產(chǎn)品名稱:Anti-Von Willebrand Factor抗體,血管假性血友病因子/血管性血友病因子抗體
產(chǎn)品編號(hào):BYK-10048R
英文名稱: Factor
中文名稱:血管假性血友病因子/血管性血友病因子抗體
英文別名:Von Willebrand Factor; Coagulation factor VIII; F8VWF; Factor VIII related antigen; von Willebrand antigen 2; Von Willebrand antigen II; Von Willebrand disease; VWD; VWF; Coagulation factor VIII; Coagulation factor VIII VWF; F8VWF; VWF_HUMAN.
產(chǎn)品規(guī)格:0.1ml/0.2ml
此抗體本公司有單抗和多抗,可用于免疫組化(IHC),免疫印跡(WB),酶聯(lián)免疫(ELISA),IF、IP、流式細(xì)胞術(shù)等實(shí)驗(yàn)(可來(lái)電索取說(shuō)明)
保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Factor抗體,血管假性血友病因子/血管性血友病因子抗體Western Blotting 具體實(shí)驗(yàn)步驟:
Western,也稱Western blot、Western blotting、Western 印跡,是用抗體檢測(cè)蛋白的重要方法之一。
Western可以參考如下步驟進(jìn)行操作:
收集蛋白樣品(Protein sample preparation)
可以使用適當(dāng)?shù)牧呀庖毫呀赓N壁細(xì)胞、懸浮細(xì)胞或組織樣品。對(duì)于某些特定的亞細(xì)胞組份蛋白,例如細(xì)胞核蛋白、細(xì)胞漿蛋白、線粒體蛋白等,可以參考相關(guān)文獻(xiàn)提取這些亞細(xì)胞組份蛋白,也可以使用試劑盒進(jìn)行抽提。
收集完蛋白樣品后,為確保每個(gè)蛋白樣品的上樣量*,需要測(cè)定每個(gè)蛋白樣品的蛋白濃度。根據(jù)所使用的裂解液的不同,需要采用適當(dāng)?shù)牡鞍诐舛葴y(cè)定方法。因?yàn)椴煌牡鞍诐舛葴y(cè)定方法對(duì)于一些去垢劑和還原劑等的兼容性差別很大。
Factor抗體,血管假性血友病因子/血管性血友病因子抗體
英文介紹:Von Willebrand Factor (VWF) was previously known as Factor VIII related antigen. VWF is synthesized exclusively by endothelial cells and megakaryocytes, and stored in the intracellular granules or constitutively secreted into plasma. This glycoprotein functions as both an antihemophilic factor carrier and a plaet vessel wall mediator in the blood coagulation system. Important in the maintenance of homeostasis, it participates in plaet vessel wall interactions by forming a noncovalent complex with coagulation factor VIII at the site of vascular injury. The Von Willebrand factor has functional binding domains to plaet glycoprotein Ib, glycoprotein IIb/IIIa, collagen and heparin. Mutations in this gene or deficiencies in this protein result in Von Willebrand's disease. VWD is characterized by frequent bleeding (gingival, minor skin quantitative lacerations, menorrhagia, etc.).
此產(chǎn)品本公司有標(biāo)記的一抗出售,相關(guān)標(biāo)記有:Alexa Fluor 350 標(biāo)記、Alexa Fluor 488 標(biāo)記、Alexa Fluor 555 標(biāo)記、Alexa Fluor 647 標(biāo)記、AP標(biāo)記、APC標(biāo)記、Biotin標(biāo)記、Cy3標(biāo)記、Cy5標(biāo)記、Cy5.5標(biāo)記、Cy7標(biāo)記、FITC標(biāo)記、Gold標(biāo)記、HRP標(biāo)記、PE標(biāo)記、PE-Cy3標(biāo)記、PE-CY5標(biāo)記、PE-CY5.5標(biāo)記、PE-CY7標(biāo)記、RBITC標(biāo)記
注意事項(xiàng):This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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