產(chǎn)品名稱:Anti-GTF2F1抗體,轉(zhuǎn)錄起始因子RAP30抗體
產(chǎn)品編號:BYK-8479R
英文名稱:Anti-GTF2F1
中文名稱:轉(zhuǎn)錄起始因子RAP30抗體
英文別名:TFIIF RAP30; ATP dependent helicase GTF2F2; BTF 4; BTF4; General transcription factor IIF polypeptide 1; General transcription factor IIF polypeptide 1 74 kDa subunit protein; General transcription factor IIF polypeptide 1 74kDa; General transcription factor IIF polypeptide 2 30kDa; General Transcription Factor IIF Polypeptide 2; GTF2F1; GTF2F1 protein; GTF2F2; RAP 30; RAP 74; RAP30; RAP74; TF2F 1; TF2F 2; TF2F1; TF2F2; TFIIF alpha; TFIIF beta; Transcription initiation factor IIF alpha subunit; Transcription Initiation Factor IIF beta Subunit; Transcription initiation factor IIF subunit beta; Transcription initiation factor RAP30; Transcription initiation factor RAP74; T2FB_HUMAN.
產(chǎn)品規(guī)格:0.1ml/0.2ml
此抗體本公司有單抗和多抗,可用于免疫組化(IHC),免疫印跡(WB),酶聯(lián)免疫(ELISA),IF、IP、流式細(xì)胞術(shù)等實(shí)驗(yàn)(可來電索取說明)
保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Anti-GTF2F1抗體,轉(zhuǎn)錄起始因子RAP30抗體英文介紹:In eukaryotic systems, initiation of transcription from protein-coding genes is a complex process requiring RNA polymerase II and broad families of auxiliary transcription factors. Such factors can be divided into two major functional classes: the basal factors that are required for transcription of all Pol II genes, including TFIIA, TFIIB, TFIID, TFIIE, TFIIF and TFIIH; and sequence-specific factors that regulate gene expression. The basal transcription factors and Pol II form a specific multiprotein complex near the transcription start site by interacting with core promotor elements such as the TATA box generally located 25-30 base pairs upstream of the transcription start site. TFIIF, a heteromer composed of a small (RAP 30) and a large (RAP 74) subunit, is required for RNA polymerase II to assemble into a preinitiation complex formed by promotor DNA and the general factors TFIID, IIA and IIB. In addition, TFIIF stimulates transcription elongation by RNA polymerase II.
此產(chǎn)品本公司有標(biāo)記的一抗出售,相關(guān)標(biāo)記有:Alexa Fluor 350 標(biāo)記、Alexa Fluor 488 標(biāo)記、Alexa Fluor 555 標(biāo)記、Alexa Fluor 647 標(biāo)記、AP標(biāo)記、APC標(biāo)記、Biotin標(biāo)記、Cy3標(biāo)記、Cy5標(biāo)記、Cy5.5標(biāo)記、Cy7標(biāo)記、FITC標(biāo)記、Gold標(biāo)記、HRP標(biāo)記、PE標(biāo)記、PE-Cy3標(biāo)記、PE-CY5標(biāo)記、PE-CY5.5標(biāo)記、PE-CY7標(biāo)記、RBITC標(biāo)記
注意事項(xiàng):This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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Anti-GTF2F1抗體,轉(zhuǎn)錄起始因子RAP30抗體抗體溶解方法及分裝:
1、購買抗體后,查看標(biāo)簽上的規(guī)格,如:0.1ml、0.2ml
2、以規(guī)格為 0.1ml 的凍干粉抗體為例(部分抗體為液體,無需再溶解):
溶解方法一:用移液器加 0.1ml 雙蒸水到試管內(nèi),混勻,-20℃保存,若為其它規(guī)格(如:0.2ml)的凍干粉抗體,則加與標(biāo)簽上規(guī)格*的(如:0.2ml)雙蒸水溶解,
混勻,-20℃保存。
溶解方法二:用移液器加 0.05ml(規(guī)格的 50%)雙蒸水到試管內(nèi),再加 0.05ml(規(guī)格的 50%)
的甘油到試管內(nèi),混勻,-20℃保存,若為其它規(guī)格(如:0.2ml)的凍干粉抗體,則加標(biāo)簽上規(guī)格的 50%(如:0.1ml)雙蒸水,再加
標(biāo)簽上規(guī)格的 50%(如:0.1ml)甘油溶解,混勻,-20℃保存。
抗體溶解后,將其分裝成 5-10 支(以 0.1ml 抗體為例,20ulx5 支或 10ulx10 支), -20℃保存,用
一支取一支,避免反復(fù)凍溶。
另:預(yù)試驗(yàn)可做幾個(gè)梯度 1:100、1:200、1:400 稀釋,如果背景高,還可再稀釋,選一個(gè)zui
佳的稀釋比例,再正式做,效果。
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