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產(chǎn)品名稱(chēng):Anti-Fascin1抗體,纖維束1抗體
產(chǎn)品編號(hào):BYK-0796R
英文名稱(chēng):Anti-Anti-Fascin1
中文名稱(chēng):纖維束1抗體
英文別名:55 kDa actin bundling protein; 55 kDa actin-bundling protein; Actin bundling protein; FAN 1; Fscin1; FAN1; Fascin 1; Fascin; Fascin homolog 1 actin bundling protein (Strongylocentrotus purpuratus); Fascin homolog 1; Fascin1; FLJ38511; FSCN 1; FSCN1; FSCN1_HUMAN; HSN; p55; Singed (Drosophila) like (sea urchin fascin homolog like); Singed drosophila homolog like; Singed like (fascin homolog sea urchin) (Drosophila); Singed like (fascin homolog sea urchin); Singed like protein; Singed-like protein; SNL; Strongylocentrotus purpuratus.
英文介紹:This gene encodes a member of the fascin family of actin-binding proteins. Fascin proteins organize F-actin into parallel bundles, and are required for the formation of actin-based cellular protrusions. The encoded protein plays a critical role in cell migration, motility, adhesion and cellular interactions. Expression of this gene is known to be regulated by several microRNAs, and overexpression of this gene may play a role in the metastasis of multiple types of cancer by increasing cell motility. Expression of this gene is also a marker for Reed-Sternberg cells in Hodgkin's lymphoma. A pseudogene of this gene is located on the long arm of chromosome 15.
產(chǎn)品規(guī)格:0.1ml/0.2ml
注意事項(xiàng):This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
此抗體本公司有單抗和多抗,可用于免疫組化(IHC),免疫印跡(WB),酶聯(lián)免疫(ELISA),IF、IP、流式細(xì)胞術(shù)等實(shí)驗(yàn)(可來(lái)電索取說(shuō)明)
此產(chǎn)品本公司有標(biāo)記的一抗出售,相關(guān)標(biāo)記有:Alexa Fluor 350 標(biāo)記、Alexa Fluor 488 標(biāo)記、Alexa Fluor 555 標(biāo)記、Alexa Fluor 647 標(biāo)記、AP標(biāo)記、APC標(biāo)記、Biotin標(biāo)記、Cy3標(biāo)記、Cy5標(biāo)記、Cy5.5標(biāo)記、Cy7標(biāo)記、FITC標(biāo)記、Gold標(biāo)記、HRP標(biāo)記、PE標(biāo)記、PE-Cy3標(biāo)記、PE-CY5標(biāo)記、PE-CY5.5標(biāo)記、PE-CY7標(biāo)記、RBITC標(biāo)記
保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Anti-Fascin1抗體,纖維束1抗體免疫組化染色:
封閉(Blocking)
轉(zhuǎn)膜完畢后,立即把蛋白膜放置到預(yù)先準(zhǔn)備好的Western洗滌液中,漂洗1-2分鐘,以洗去膜上的轉(zhuǎn)膜液。從轉(zhuǎn)膜完畢后所有的步驟,一定要注意膜的保濕,避免膜的干燥,否則極易產(chǎn)生較高的背景。
用微型臺(tái)式真空泵吸盡洗滌液,加入Western封閉液,在搖床上緩慢搖動(dòng),室溫封閉60分鐘。對(duì)于一些背景較高的抗體,可以在4℃ 封閉過(guò)夜。
一抗孵育(Primary antibody incubation)
參考一抗的說(shuō)明書(shū),按照適當(dāng)比例用Western一抗稀釋液稀釋一抗。
用微型臺(tái)式真空泵吸盡封閉液,立即加入稀釋好的一抗,室溫或4℃在側(cè)擺搖床上緩慢搖動(dòng)孵育一小時(shí)。如果一抗孵育一小時(shí)效果不佳,可以在4℃緩慢搖動(dòng)孵育過(guò)夜。回收一抗。加入Western洗滌液,在側(cè)擺搖床上緩慢搖動(dòng)洗滌5-10分鐘。吸盡洗滌液后,再加入洗滌液,洗滌5-10分鐘。共洗滌3次。如果結(jié)果背景較高可以適當(dāng)延長(zhǎng)洗滌時(shí)間并增加洗滌次數(shù)。
二抗孵育(Secondary antibody inucubation)
參考二抗的說(shuō)明書(shū),按照適當(dāng)比例用Western二抗稀釋液稀釋辣根過(guò)氧化物酶(HRP)標(biāo)記的二抗。
用微型臺(tái)式真空泵吸盡洗滌液,立即加入稀釋好的二抗,室溫或4℃在側(cè)擺搖床上緩慢搖動(dòng)孵育一小時(shí)。回收二抗,加入Western洗滌液,在側(cè)擺搖床上緩慢搖動(dòng)洗滌5-10分鐘。吸盡洗滌液后,再加入洗滌液,洗滌5-10分鐘。共洗滌3次。如果結(jié)果背景較高可以適當(dāng)延長(zhǎng)洗滌時(shí)間并增加洗滌次數(shù)。
蛋白檢測(cè)(Detection of proteins)
參考相關(guān)說(shuō)明書(shū),使用BeyoECL, Western 熒光檢測(cè)試劑等ECL類(lèi)試劑來(lái)檢測(cè)蛋白。
洗片時(shí)可以使用X光片自動(dòng)洗片機(jī)。如果沒(méi)有自動(dòng)洗片機(jī),可以用顯影定影試劑盒自行配制顯影液和定影液進(jìn)行手工洗片。
膜的重復(fù)利用(Membrane recovery)
如果蛋白樣品非常寶貴,可以使用Western一抗二抗去除液處理蛋白膜,以重復(fù)利用蛋白膜。
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